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《Shandong Agricultural Sciences》 2014-06
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Pathogen Identification of Anthracnose and Leaf Mildew from Anthurium andraeanum as Cut Flower

Li Li;Liu Huixiang;Guo Xianfeng;Meng Fanzhi;College of Forestry,Shandong Agricultural University;College of Plant Protection,Shandong Agricultural University;  
Anthracnose and leaf mildew are major diseases which harm the production of cut flower Anthurium andraeanum. The pathogen of the two diseases was identified through traditional morphological method combined with molecular biological method. From the infected plants,the pathogenic strains were obtained through the conventional organization separation,and the morphological observation and pathogenicity identification were conducted. The molecular identification was also conducted by ITS sequence analysis and comparison. The results suggested that Colletotrichum gloeosporioides was the pathogen of Anthurium andraeanum anthracnose,which had the highest homology( more than 99%) in ITS sequence with KC172072. 1 found from GenBank. The pathogenic bacteria of Anthurium andraeanum leaf mildew were Cladosporium cladosporioides and Alternaria alternata,which had the highest homology with KC880082. 1 and JX406531. 1 found from GenBank respectively; the homology reached to 96% and 97% respectively. The molecular identification indicated the same results with that of morphological identification.
【Fund】: 国家林业局“948”引进项目(2012-4-57)
【CateGory Index】: S436.8
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