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《SHANDONG AGRICULTURAL SCIENCES》 1996-06
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Isolation and Purification of Infectious Bursal Disease Virus and Preparation of Antiserum

Ma Xiaoli, Zhao Hui, Mao Bingyu & Zhang Hongwei (Development Institute of Life Science Academy, Shandong University, Jinan 250100)  
To get pure infectious bursal disease virus (IBDV) should be done as followed: culture IBDV with allantoic fluid of chicken embryo, centrifuge the fluid at high speed, precipitate it with PEG, putting sucrose at the bottom to centrifuge it again at ultra-high speed, and at last do NaBr density gradient centrifugation. Under JEM-100CX, the virus particle was observed in six-angle shape, free from brain, with diameter being about 60 nm and the buoyant density 1.30-1.36 g/ml, the virus content averaged 1.72 mg/ml. Immuning this virus to rabbit can produce antiserum with 1∶32 of valence, showing high purity and specificity.
【Fund】: 国家教委博士点基金
【CateGory Index】: S858.310.25
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【References】
Chinese Journal Full-text Database 2 Hits
1 CAO Kang 1, ZHANG Wei-dong 2, SHI Qiao-fa 1,LI Hong 1,JIANG Zhong-hua 1, LI Ming-yuan 1 (1. Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China; 2. Department of Pathobiology, the School of Basic Medical Sciences,Hebei Medical University, Shijiazhuang 050017,China);COMPARISON OF TWO METHODS FOR PURIFICATION OF INFLUENZA A VIRUS[J];Journal of Hebei Medical University;2005-03
2 ZHU Liqian~1 YI Minglin~4 XI Jun~3 ZHAO Xuyong~1 WANG Li~4 LI Qingzhou~1 ZHANG Gaiping~4 (1.Henan Agricultrual University,Zhengzhou Henan 450002,China; 2.Henan Academy of Agriculture Science Key Laboratory of New Fuchsin Zhengzhou Henan 450002,China; 3.Jilin University,Changchun Jilin ll3006,China; 4.Henan Provincial Key Laboratory of Animal Immunology,Zhengzhou Henan 450002,China);Optimization of the condition of using immunohistochemistry to dedect the IBDV infected CEF in quantitative relatively[J];Journal of Zhengzhou College of Animal Husbandry Engineering;2008-02
【Co-references】
Chinese Journal Full-text Database 2 Hits
1 Pan Saiyi Huang Jiankang Zhou Jiexie (Institute of Parasitic Diseases of Guangdong Province, Guangzhou 510300) Wu Jianmin (Department of Epidemiology, Hunan Medical University, Changsha 410078);ESTABLISHMENT OF HYBRIDOMA TECHNIQUE AND STUDY ON FUSION CONDITIONS OF HYBRIDOMA CELLS[J];BULLETIN OF HUNAN MEDICAL UNIVERSITY;1997-02
2 YIN Quan-zhang YAO Kun WANG Fang XU Wen-rong XU jian CHEN Yun ZHOU Feng FENG Dong-ju (Department of Microbiology and Immunology, NJMU, Nanjing 210029, China);Cloning,expression and purification of human herpesvirus 6 U94 and its antibodies production[J];Acta Universitatis Medicinalis Nanjing(Natural Science);2007-08
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 WU Sheng-xi,LI Qing-ji,LU Ning,LU Rui,CAI Jia-li(School of Bioengineering,Chongqing Institute of Technology,Chongqing 400050,China);Purification of Newcastle Disease Nirus(NDV) with Absorption and Release-RBC Method[J];Journal of Chongqing Institute of Technology(Natural Science Edition);2007-11
2 JIANG Feng-ying,HE Xi-zhong,WANG Ying,LI Chun-hua,QIAN Yong-qing,ZOU Yong (Animal Husbandry and Veterinary Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai,201106,China);Ultra Centrifugation the Cell Cultures of TGEV and RT-PCP[J];Progress in Veterinary Medicine;2007-04
3 Lu Cui,MA Xiao-ming,YIN Yan-bo,SHAN Hu(College of Animal Science,QAU,Qingdao 266109,China);Isolation and Identification of H1 Subtype Swine Influenza Virus[J];Journal of Qingdao Agricultural University(Natural Science);2009-01
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