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Isolation and Purification of Infectious Bursal Disease Virus and Preparation of Antiserum

Ma Xiaoli, Zhao Hui, Mao Bingyu & Zhang Hongwei (Development Institute of Life Science Academy, Shandong University, Jinan 250100)  
To get pure infectious bursal disease virus (IBDV) should be done as followed: culture IBDV with allantoic fluid of chicken embryo, centrifuge the fluid at high speed, precipitate it with PEG, putting sucrose at the bottom to centrifuge it again at ultra-high speed, and at last do NaBr density gradient centrifugation. Under JEM-100CX, the virus particle was observed in six-angle shape, free from brain, with diameter being about 60 nm and the buoyant density 1.30-1.36 g/ml, the virus content averaged 1.72 mg/ml. Immuning this virus to rabbit can produce antiserum with 1∶32 of valence, showing high purity and specificity.
【Fund】: 国家教委博士点基金
【CateGory Index】: S858.310.25
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1 CAO Kang 1, ZHANG Wei-dong 2, SHI Qiao-fa 1,LI Hong 1,JIANG Zhong-hua 1, LI Ming-yuan 1 (1. Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China; 2. Department of Pathobiology, the School of Basic Medical Sciences,Hebei Medical University, Shijiazhuang 050017,China);COMPARISON OF TWO METHODS FOR PURIFICATION OF INFLUENZA A VIRUS[J];Journal of Hebei Medical University;2005-03
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1 WU Sheng-xi,LI Qing-ji,LU Ning,LU Rui,CAI Jia-li(School of Bioengineering,Chongqing Institute of Technology,Chongqing 400050,China);Purification of Newcastle Disease Nirus(NDV) with Absorption and Release-RBC Method[J];Journal of Chongqing Institute of Technology(Natural Science Edition);2007-11
2 JIANG Feng-ying,HE Xi-zhong,WANG Ying,LI Chun-hua,QIAN Yong-qing,ZOU Yong (Animal Husbandry and Veterinary Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai,201106,China);Ultra Centrifugation the Cell Cultures of TGEV and RT-PCP[J];Progress in Veterinary Medicine;2007-04
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