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《SHANDONG AGRICULTURAL SCIENCES》 1998-04
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Genetic Analysis on the Progeny of the Transgenic Tomato with Double-resistance to TMV and CMV

Shan Lei,Bi Yuping,Wang Xingjun,Xu Pingli & Li Guangcun (Biotechnology Research Center,Shangdong Academy of Agricultural Sciences,Jinan 250100)  
The progeny in both T 1 and T 2 generation of the transgenic tomato containing TMV and CMV coat protein chimaeric gene had been analyzed through PCR,Southern blot,and the detection of resistance to viruses in green house and fields.The results showed that the segregation pattern of the TMV and CMV coat protein chimaeric gene,which has been integrated into one tomato plant,is accordant with Mendel's law of segregation,namely,the segregation ratio is 3∶1 in T 1 generation,and most of transgenic tomato lines,except a few lines keeping genetic segregation,remain genetic stability in T 2 generations.In this study,three desirable transgenic tomato lines with genetic stability has been obtained.
【Fund】: 山东省科委下达课题
【CateGory Index】: S641.203
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