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《Journal of Anhui University(Natural Sciences)》 1988-03
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Isolation and Purification of Glucoamylase from Aspergillus niger UV-11 and prelimianry Study Multiple Forms of Glucoamylase

Wu Zhifang, Wu Anzheng, Huang Ruduo, Fang Yuke  
Glueoamylase was isolated and purified from commercial products of As pergillus niger mutant strains UV—11 has been shown to exist in multiple orms, seperable by polyacrylamide gel(7.5%)electrophoresis as nearly ocated bands. After ammonium sulfate fractionation at 60% saturated gel filtration (Sep hadexG—25)and DEAE—Cellulose DE_52 column chromatography, fou fractions(peak Ⅰ—Ⅳ)from the eluate were obtained.The four fractions have been purified 0.92—1.84 fold with a total recovery of 26.6%. Both protein and carbohydrate of crude enzyme and purified fractionsr were tested in PAGE, date showed that the Glucoamylase from A.niger mutant strains UV—11 was a glycoprotein.
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 WU WU-TONG YU PIN-HUA XIA ER-NING SHI WEI-XING XU BI-RU CHEN QIONG-HUA Nanjing College of Pharmacy;ISOLATION AND PURIFICATION OF POLYSACCHARIDES TF-A,TF-B AND TF-C FROM THE SPORES OF TREMELLA FUCIFORMIS BERK AND DETERMINATION OF THEIR MOLAR RATIOS[J];生物化学与生物物理学报;1984-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIN Ai qin1,FANG YouLan2,LIN Xi2,LU Wei2(1.Fuqing Branch of Fujian Normal University,Fuqing,Fujian,350300,China 2.Chemistry and Material College Fujian Normal University,Fuzhou,Fujian,350007,China);Component Analysis of Polysaccharide from Opuntia and Determination of its Molar Ratio[J];Journal of Fuqing Branch of Fujian Normal University;2006-02
2 Lin Yuman(Experiment Center) Yan Chunsheng(Department of Physical Education) Yu Ping(Institute of Polymer Science);Studies on Soluble Polysaccharide by Acid Extraction from Dictyophora duplicata——Isolation,Purification and Identification of Dd—2DE[J];JOURNAL OF FUJIAN TEACHERS UNIVERSITY(NATURAL SCIENCE);1998-02
3 Ke Huo-Zhao1,Lin Yu-man2 (1.Fujian Pharmaceutial School,Fuzhou ,350002; 2.Experiment Center,Fujian Normal University,Fuzhou,350007);Studies on Component analysis and antitumour of glycoprotein DiGP-2 from the Submerged mycelium of Dityophora indusiata Fisch[J];Strait Pharmaceutical Journal;2001-04
4 WANG Wen-zheng1,2,L Jian-guo1,2,HE Bao-hua1(1.Gansu Academy of Membrane Science and Technology,Lanzhou 730020,China;2.Gansu Research Center of Membrane Separation Engineering Technology,Lanzhou 730020,China);Application of Ultrafiltration Membrane Technique in Polysaccharides[J];Chemistry & Bioengineering;2011-05
5 Guo Yumei1,2 Yang Shizhao2 Cheng Caifa1 Guo Yujie3 (1.Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province,Xuzhou Normal University,Jiangsu Xuzhou 221116;2.Department of Chemistry,Xuzhou Normal University,Jiangsu Xuzhou 221116;3.Engineer College of Animal Husbandry and Veterinary Science,Henan Agricultural University,Henan Zhengzhou 450002);Optimum Selection of Carbon and Nitrogen Sources pH for Submerged fermentation of Fuscoporia Punctata Cunn[J];Chemical Industry Times;2007-07
6 LIU Chun-hui, Xi Tao , Lin Qin-xiong, Gao Yi, Xing Ying-ying(School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China);Extraction, purification and immunological activity assay of a polysaccharide from eggs of sea urchin Strongylocentrotus nudus[J];Chinese Journal of Marine Drugs;2006-03
7 TIAN Long(Institute of Life Science and Technology,Nanyang Normal College,Nanyang 473061,China);Extraction,Purification and Structural Analysis of Alkali-soluble Polysaccharide in Actinidia chinensis Planch.[J];Journal of Huazhong Agricultural University;2007-03
8 Yang Juan\ Wu Moucheng\ Zhang Shenghua\= (Huazhong Agricultural University , Wuhan 430070) \;;STUDY ON ISOLATION,PURIFICATION AND CHARACTERISTICS OF POLYSACCHARIDE Le FROM THE FRUITBODY OF Lentinus Edodes[J];JOURNAL OF HUAZHONG AGRICULTURAL;1999-02
9 XIA ER-NING CHEN QIONG-HUA (Division of Biochemistry, China Pharmaceutical University, Nanjing);ISOLATION, ANALYSIS AND BIOLOGICAL ACTIVITIES OF THE POLYSACCHARIDE OF TREMELLA FUCIFORMIS BERKELEY[J];;1988-03
10 WANG Zhongmin, WU Moucheng, YIN Jingzhang. LI Xiaoding(1.College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;2.Xinjiang Agricultural University,Wulumuqi 830052,China);The isolation of VLP-2 and the analysis of its composition of monosaccharide[J];Journal of Henan Agricultural University;2003-01
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 WU Wu-tong CHEN Qiong-hua (Nanjing College of Pharmacy) XU Bi-ru WANG Sai-zhen HHEN Jin-tu (Sanming Fungi Research Institute in Fujian, Sanming);ISOLATION AND ANALYSIS OF POLYSACCHARIDE FROM THE SPORES OF TREMELLA FUCIFORMIS[J];Mycosystema;1982-02
2 Ye Chunqu and Fang Jinian(Shanghai Institute of Materia Medica, Academia Sinica);STUDIES ON THE CONSTITUENTS OF POLYSACCHARIDES SB-Ⅰ AND SB-Ⅱ FROM SHIRAIA BAMBUSICOLA——Ⅰ. IDENTIFICATION OF POLYSACCHARIDES SB-Ⅰ AND SB-Ⅱ FROM SHIRAIA BAMBUSICOLA AND DETERMINATION OF THEIR MOLAR RATIOS[J];Acta Pharmaceutica Sinica;1981-07
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