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《Journal of Anhui University(Natural Sciences)》 2001-03
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Studies on the preparation and fusion of protoplast from Lentinula edodes hyphae

LI Rui,MA Shou-neng,LIU Qun,WU Ke,LIU Bin,PAN Ren-rui (The Chemistry and Biotechnology Department of Hefei Union University, Hefei 230022,China)  
The influence of different cell age, enzymolysis time, temperature and osmotic pressure stabilizer on productive rate of protoplast from Lentinula edodes were discussed in this paper. It is the best conditions of preparing protoplast with Lentinula edodes hyphae that cell age was 6 days, That enzymolysis time was 3 hours, that enzymolysis temperature was 34℃ and that concentration of mannitol as osmotic pressure stabilizer was 0.6mol/l. In addition, the protoplast from Lentinula edodes B01and L26 were fused with 35% polyetlylene glycol (PEG) solution as fusion inducer and formation of fusant was observed under the microscope.
【Fund】: 合肥联合大学科研基金资助项目 (2 0 0 0 12 )
【CateGory Index】: S646
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