Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Anhui University(Natural Sciences)》 2001-03
Add to Favorite Get Latest Update

Studies on the preparation and fusion of protoplast from Lentinula edodes hyphae

LI Rui,MA Shou-neng,LIU Qun,WU Ke,LIU Bin,PAN Ren-rui (The Chemistry and Biotechnology Department of Hefei Union University, Hefei 230022,China)  
The influence of different cell age, enzymolysis time, temperature and osmotic pressure stabilizer on productive rate of protoplast from Lentinula edodes were discussed in this paper. It is the best conditions of preparing protoplast with Lentinula edodes hyphae that cell age was 6 days, That enzymolysis time was 3 hours, that enzymolysis temperature was 34℃ and that concentration of mannitol as osmotic pressure stabilizer was 0.6mol/l. In addition, the protoplast from Lentinula edodes B01and L26 were fused with 35% polyetlylene glycol (PEG) solution as fusion inducer and formation of fusant was observed under the microscope.
【Fund】: 合肥联合大学科研基金资助项目 (2 0 0 0 12 )
【CateGory Index】: S646
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 YU Min,SHEN Ye-shou (School of Life Science,Anhui University,Hefei 230039,China);Comparision of polysaccharide and its immunological activity from the different rhizomorph of Armillaria Mellea[J];Journal of Anhui University(Natural Sciences);2002-01
Chinese Journal Full-text Database 1 Hits
1 LI Gang;LI Baojian (Biotechnology Research Center, Zhongshan University, Guangzhou 510275);STUDY ON THE SEPARATION AND REGENERATION OF GANODERMA PROTOPLAST[J];MYCOSYSTEMA;1999-01
Chinese Journal Full-text Database 10 Hits
1 WANG Qian et al(College of Life Science,Hebei University,Baoding,Hebei 071002);Study on the Thermotolerance of Pleurotus ostreatus Cell Engineering Strains[J];Journal of Anhui Agricultural Sciences;2007-05
2 LI Shou-mian et al (College of Horticulture,Agriculture University of Hebei,Baoding,Hebei 071001);Application of Protoplast Technology of Edible Mushroom[J];Journal of Anhui Agricultural Sciences;2007-25
3 LI Jing et al(College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071001);Mycelium Growth of Parent Strains and Mutation Hybrid Progeny of Pleurotus ferulae Lenzi and its Comparison and Analysis of Esterase Isozyme[J];Journal of Anhui Agricultural Sciences;2007-29
4 MENG Yanqiong1,SONG Min2,PENG Fan1,FAN Meizhen1 (1College of Forest Utilization,Anhui Agricultural University,Hefei 230036; 2Agricultural and Forest Bureau of Bengbu City);Studies on Submerged Fermentation Process of Pleurotus eryngii[J];Journal of Anhui Agricultural University;2003-03
5 PU Shun-chang,LI Chun-ru,WANG Xiao-yan,HUANG Bo,FAN Mei-Zhen,LI Zeng-zhi(Provincial Key Laboratory of Microbial Pest Control,Anhui Agricultural University,Hefei 230036);Preparation of protoplast from Paecilomyces tenuipes,the anamorph of Cordyceps takaomontana[J];Journal of Anhui Agricultural University;2008-03
6 Bao Zenghai;On Preventive Measures of Some Organic Characterized Diseases[J];Journal of Chengde Vocational and Technical College for Nationalitiese;2001-02
7 MA Ji-fang,FAN Yong-shan,ZHANG Jing-bai,DONG Jin-gao (Mycotoxin Laboratory, Agricultural University of Hebei, Baod ing 071001, China);Isolation of protoplasts from Exserohilum turcicum[J];Journal of Agricultural University of Hebei;2003-03
8 LI Nan,XU Xiuhong (College of Resources and Environmental Sciences,Northeast Agricultural University,Harbin 150030,China);Protoplast preparation and regeneration of Auricularia auricula 29[J];Journal of Northeast Agricultural University;2009-07
9 Wang Zhiyong Li Xiaohong(Xianning Education College of Hubei Xianning 437100);Primary Studying Report on Adaptation of Lactarius Hatsidake Tanaka to Environmental Condition[J];Hubei Forestry Science and Technology;2004-04
10 LIN Yan\|quan 1, LI Kai\|ben 1, LIN Xing\|sheng 1, HUANG Jian\|cheng 2, CHEN Ti\|qiang 1, YU Ying\|rui 1, HU Run\|fang 2 (1.Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou,Fujian\ 350013, China; 2.Institute of C;Cultivation comparison and esterase isozyme analysis of Agaricus sylvaticus and Agaricus blazil[J];Fujian Journal of Agricultural Sciences;2001-02
Chinese Journal Full-text Database 10 Hits
1 JIANG Li~1,KONG Xiao-wei~2,WU Xiao-jie~1,LI Shao-song~1(1.School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China;2.School of Life Science,Anhui University,Hefei 230039,China);Isolation and purification of protoplasts from tobacco leaves[J];Journal of Anhui University(Natural Sciences);2006-06
2 HAN Feng-min et al(College of Life Science,Nanjing Normal University,Nanjing,Jiangsu 210046);Screening and Identification of a Halobacteria Strain Producing Amylase with Saline-Alkali and High Temperature Tolerance[J];Journal of Anhui Agricultural Sciences;2008-15
3 LI Xiang-kai,AN Zhi-dong, ZHU Fei (College of Life Sciences, Wuhan University, Wuhan 430072);Study on Fusion of Biparental Inactivated Protoplasts of Streptomyces lincolnensis[J];Amino Acids & Biotic Resources;2001-04
4 LI Ping1,ZHANG Qiang1,WAN Xiaochun1,TAO Wenyi2,DING Xiaoling2 Biotechnology of Ministry of Agriculture,Hefei 230036; 2.Southern Yangtze University);Effects of the Protoplast Formation and Regeneration from Aspergillus niger[J];Journal of Anhui Agricultural University;2003-03
5 PU Shun-chang,LI Chun-ru,WANG Xiao-yan,HUANG Bo,FAN Mei-Zhen,LI Zeng-zhi(Provincial Key Laboratory of Microbial Pest Control,Anhui Agricultural University,Hefei 230036);Preparation of protoplast from Paecilomyces tenuipes,the anamorph of Cordyceps takaomontana[J];Journal of Anhui Agricultural University;2008-03
6 WANG Jun~(1,2),XIE Hao~(3),GUO Er-hu~(2),LI Ai-jun~(2),FAN Hui-ping~(2)(1.China Agricultural university,Beijing 100094;2.Institue of Foxtail Millet, Shanxi Academy of Agricultural Sciences,ChangZhi 046011;3.Beijing Agricultural College, Beijing 102206);DNA Molecular Marker and Its Application on the Heredity and Breeding Research in Foxtail Millet[J];Journal of Beijing Agricultural College;2005-01
7 ;Studies on Isolation and Regeneration of Protoplast from Tremella Fuciformis Mycelium[J];Journal of Changjiang Vegetables;2001-09
8 Liu Deshao,Zheng Qiang (Chongqing Environmental Protection Bureau,Chongqing 400015);Study on Environmental Biological Technology and Its Application to Water Pollution Control[J];Chongqing Environmental Science;2001-01
9 Xu Yanbin 1,Jiang Xia 2 (1.Guangdong University of Technology,Guangzhou 510090; 2.Guangdong Research Institute of Food Industry,Guangzhou 510315);Study on the Construction of Engineering Bacteria Degrading Organic Chloride[J];Chongqing Environmental Science;2001-02
10 Guo Jianchun(Department of Biology and geography);THE STUDY OF PROTOPLAST'S FUSION AND CROSSBREEDING OF SPECIES IN PLEUTOTUS[J];;1994-03
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 KONGXiao wei,YUMin ,ZHAXiang dong (SchoolofLifeScience ,AnhuiUniversity ,Hefei 2 30 0 39,China);Study on esterase isoenzymese(EST) in Armillaria mellea (Am)[J];Journal of Anhui University(Natural Sciences);2004-02
2 WANG Chun-ping1,2,WEI Qiang1,BAO Guo-lian1,LIU Yan1,SHAO Ze-xiang1,2,JI Quan-an1(1.Animal Husbandry and Veterinary Institute,Zhejiang Academy of Agricultural Sciences,Hangzhou,Zhejiang,310021,China;2.College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Tai'an,Shandong,271018,China);Progress on Microbial Protoplast Fusion Technology[J];Progress in Veterinary Medicine;2008-05
3 SONG Cheng-zhi et al (Department of Chemistry,Zhaotong Teacher’s College,Zhaotong,Yunnan 657000);Chemical Constituents and Pharmacological Function of Armillaria mellea[J];Journal of Anhui Agricultural Sciences;2010-10
4 Yuan Yuan,Liu Jingsheng,Cai Dan (College of Quartermaster Technology,Jilin University,Changchun,Jilin 130062,China);Recent Research on Chemical Composition and Pharmacological Function of Armillaria Mellea[J];Academic Periodical of Farm Products Processing;2008-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved