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《Journal of Anhui Polytechnic University》 2011-03
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Compound mutation screening of γ-aminobutyric acid high-yield strain

LIU Hui,ZHANG Qing-qing,LV Wen-wen(Engineering Technoligy Research Center of Microbial Fermaentation of Anhui Province, Anhui Polytechnic University,Wuhu 241000,China)  
Monascus was used as the starting strain and the protoplasm underwent UV and 5-fluorouracil complex mutation treatment,and a high-yield γ-aminobutyric acid of Monascus strain ZL307 was obtained.The results showed that after UV irradiation for 150 seconds and 0.3 mg/mL 5-fluorouracil treatment for 15 min,the yield of γ-aminobutyric acid was 491.1 mg/L.The production was improved by 60% compared with that of original strain.The production level of this mutant is stable after 10 continuous generations of passage.
【Fund】: 芜湖市重点科技计划基金资助项目(芜科计字[2009]190号文)
【CateGory Index】: TQ921.4
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