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《Journal of Anhui Polytechnic University》 2011-04
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Effect of plant hormoneon in liquid and soild medium on callus induction of solanum tuberosum

CHAI Rui-juan,YIN Hong-fei(Coll.of Bioch.Engn.,Anhui Polytechnic University,Wuhu 241000,China)  
Callus of Solanum tuberosum was induced when the culture manner was different.The culture manner means that NAA and 6-BA were added into liquid or soild medium.The results show,induction rate of callus increased firstly,and then reduced when concentration of NAA increased.Whether callus was induced in liquid or soild medium.The best induction rare of callus when cultured in liquid medium was 77.78%(NAA+6-BA:2.5 mg/L+0.5 mg/L);The best induction rate of callus when cultured in soild medium was 100.00%(NAA+6-BA:3.5 mg/L+0.5 mg/L).At the same time,when NAA and 6-BA were added into soild medium noly,induction rate of callus was higer than in liquid medium.So it was not good for induction rate of callus when NAA and 6-BA were in liquid medium.It was found that NAA can obviously influence callus induction than 6-BA.In the end,SEM was conducted on undifferentiated callus and callus differentiated roots of Solanum tuberosum.
【Fund】: 安徽高校自然科学研究基金资助项目(kj2009b123Z)
【CateGory Index】: S532
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