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《Anhui Forestry Science and Technology》 2011-01
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RP-HPLC Method for Determination of Oleanolic Acid and Ursolic Acid in Chaenomeles speciosa

QU Xiao-qing1,YI Fang2,NIE Yun1,LI Jin-li1,ZHANG Yue-wen1,DING Zhi-en1(1.Anhui Agricultural University,Hefei 230036;2.Fuyang Product Quality Supervision and Testing Institute)  
A new RP-HPLC method for determination of oleanolic acid and ursolic acid in Chaenomeles speciosa was developed.A Phenomenex C18(250 mm×4.6 mm i.d.,5 μm) column was applied;acetonitrile-water(95:5,v/v) was used as the mobile phase at a flow rate of 0.5 mL·min-1 and monitoring wavelength was at 210 nm.Linear correlation(R2 0.99) of the peak area and the concentration of the two acids ranged from 0.4 to 2 μg was obtained.The relative standard deviation(RSD) of oleanolic acid and ursolic acid were found to be 0.42% and 1.2% respectively.Analysis of laboratory-made mixtures containing known concentrations of oleanolic acid and ursolic acid of Chaenomeles speciosa,as quality control samples,gave a mean recovery percentage of 98.6% and 96.9% respectively.This method is simple,accurate and reproducible.It has wide linear range and can be used for the simultaneous determination of oleanolic acid and ursolic acid in Chaenomeles speciosa.
【Fund】: 安徽省“十一五”攻关项目(编号:08010302151)
【CateGory Index】: TS255.7
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