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《Anhui Forestry Science and Technology》 2011-01
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RP-HPLC Method for Determination of Oleanolic Acid and Ursolic Acid in Chaenomeles speciosa

QU Xiao-qing1,YI Fang2,NIE Yun1,LI Jin-li1,ZHANG Yue-wen1,DING Zhi-en1(1.Anhui Agricultural University,Hefei 230036;2.Fuyang Product Quality Supervision and Testing Institute)  
A new RP-HPLC method for determination of oleanolic acid and ursolic acid in Chaenomeles speciosa was developed.A Phenomenex C18(250 mm×4.6 mm i.d.,5 μm) column was applied;acetonitrile-water(95:5,v/v) was used as the mobile phase at a flow rate of 0.5 mL·min-1 and monitoring wavelength was at 210 nm.Linear correlation(R2 0.99) of the peak area and the concentration of the two acids ranged from 0.4 to 2 μg was obtained.The relative standard deviation(RSD) of oleanolic acid and ursolic acid were found to be 0.42% and 1.2% respectively.Analysis of laboratory-made mixtures containing known concentrations of oleanolic acid and ursolic acid of Chaenomeles speciosa,as quality control samples,gave a mean recovery percentage of 98.6% and 96.9% respectively.This method is simple,accurate and reproducible.It has wide linear range and can be used for the simultaneous determination of oleanolic acid and ursolic acid in Chaenomeles speciosa.
【Fund】: 安徽省“十一五”攻关项目(编号:08010302151)
【CateGory Index】: TS255.7
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Chinese Journal Full-text Database 10 Hits
1 Sheng Linghui, Gao Yunhua, Wang Jing, Zhao Zhengyi (National Institute of Metrology, Beijing 100013, China);DETERMINATION OF OLEANOLIC ACID AND URSOLIC ACID IN CHINESE JUJUBE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH SOLID-PHASE EXTRACTION[J];Chemical Analysis and Meterage;2008-04
2 Liu Dan~ 1,2 , Meng Yanqiu~ 2* , Zhao Juan~2(~1College of Pharmaceuticals & Biotechnology,Tianjin University, Tianjin 300072; ~2Faculty of Pharmaceutical Engineering, Shenyang Institute of Chemical Technology, Shenyang 110142);Recent Advance in the Study on Derivatives of Oleanolic Acid and Ursolic Acid[J];Chemistry;2007-01
3 ZHOU Gen-Tu(Forestry Department of Anhui, Hefei 230001, China);High-yield Culture Technology of Chaenomeles speciosa[J];Economic Forest Researches;2003-04
4 TANG Qiang1,SONG She-wu2,CHEN Hong-wei2,HUANG Bo1,SONG Li-Hua3(1.Anhui Agricultural University,HeFei,230036,China;2.Anhui Anke Biotechnology Co.Ltd.,HeFei,230088,China; 3 Xuzhou Institute of Technology,Xuzhou 221008,China);Determination of the Rutin in Chaenomeles Speciosa S. Nakai by HPLC[J];Xuzhou Institute of Technology;2007-10
5 Zhang Gui-min,Sheng Xia,Wen Yuan-yuan,Liu Su-guo,Ding Zhi-en Anhui Agriculture University (Hefei 230036);The Method of Establishing RP-HPLC to Determine Oleanolic Acid in Xuan Papaya[J];The Food Industry;2010-01
6 WANG Han-ping,WANG Li-zhi (Department of Life Sciences, Shaanxi Institute of Education, Xi'an 710061, China);Research Progress of Oleanolic Acid in Chinese Flowering Quince[J];Food Science;2007-10
7 WANG Dai-jie1,WANG Xiao1,GENG Yan-ling1,LI Sheng-bo2 (1. Shandong Analysis and Test Center,Jinan 250014,China; 2. Shandong Yate Eco-tech Co. Ltd.,Linyi 266071,China);Determination of Oleanolic Acid and Ursolic Acid in Different Species of Fructus chaenomeles by RP-HPLC[J];Food Science;2008-10
8 YAN Rui-wen,DING Yi (Modern Experiment Technology Center of Anhui University,China National Metrology Accredited Laboratory,Hefei 230039,China);Determination of flavone in Chinese Chaenomeles speciosa(Sweet) Nakai at Xuancheng[J];Journal of Biology;2008-03
9 Sun Lianna and Hong Yongfu School of Pharmacy,the Second Military Medical University of PLA. Shanghai 200433;Chemical Constituents of Chaenomeles sinensis (Thouin.) Koehne[J];中国药学(英文版);2000-01
10 XIAO Mei-tian~(1,2) YE Jing~2 HUANG Ya-yan~2 LIU Qing~2 1.Institute of Pharmaceutical Engineering,Huaqiao University,Quanzhou 362021,China; 2.Department of Chemical and Pharmaceutical Engineering,Huaqiao University,Quanzhou 362021,China;MECC determination of ursolic acid and oleanolic acid in leaf of Eriobotrya japonica[J];Chinese Journal of Pharmaceutical Analysis;2007-05
Chinese Journal Full-text Database 10 Hits
1 WANG Yun-liang1,ZHANG Fen2,ZHANG Hua1,BAI Zhi-chuan1*(1.College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China;2.School of Pharmaceutical Sciences,Southwest University,Chongqing 400715,China);Comprehensive Development and Utilization of Medicinal Chaenomeles speciosa[J];Journal of Hebei Agricultural Sciences;2010-06
2 YANG Song-jie(College of Agriculture and Life Sciences,Ankang University / Selenium-Enriched Food Engineering Key Lab of Shaanxi,Ankang, 725000,Shaanxi,China);Research Advances on Plant Germplasm Resources of Chaenomeles[J];Hubei Agricultural Sciences;2011-20
3 XIAO Shuo,DING Zhuo-ling(The Affiliated Jingzhou Hospital,Tongji Medical School,Huazhong University of Science and Technology,Jingzhou 434020,Hubei,China);Research Progress on the Exaction of Papaya Active Ingredients[J];Hubei Agricultural Sciences;2012-05
4 WU Xian,CHEN Guan-hua,WANG Kun,SHI Jie,WU Chuan-qin(College of Food and Bioengineering,Jiangsu University,Zhenjiang Jiangsu 212013,China);Determination of stilbene glycoside,emodin and chrysophanol in radix polygoni muliflori by micellar electrokinetic capillary chromatography[J];Journal of Hebei University of Science and Technology;2011-05
5 HAN Feng-mei,ZHANG Xiao-lei,CHEN Yong(Hubei Key Lab of Biotechnology of Traditional Chinese Medicine,Hubei University,Wuhan 430062,China);The inhibitory action of lamivudine combined with oleanolic acid or ellagic acid against hepatitis B virus in vitro[J];Journal of Hubei University(Natural Science);2009-04
6 Ma Xiong,Tao Liangliang,Liu Ketong,Liu Junhai (College of Chemical & Environment Science,Shaanxi University of Technology,Hanzhong 723001,China);Ultrasonic wave assisted extraction process of oleanolic acid from pomegranaterRind[J];Chemical Technology Market;2010-09
7 WANG Li-cong (Department of Chemical and Textile, Jingyin Polytechnic College, Wuxi 214405, China);Simultaneous Determination of Ursolic acid and Oleanolic acid in Hippophae rhamnoides with HPLC[J];Hebei Chemical Engineering and Industry;2009-02
8 XIE Ying,WANG Xue-yan,ZHANG Ji-gang(Tea and Food Science and Technology College of Anhui Agricultural University,Hefei,Anhui 230036);Extraction and Antimicrobial Activity of the Components from Papaya[J];Heilongjiang Agricultural Sciences;2011-09
9 REN Yi-jun,LI Hua-rong,GAO Feng-xi Tongji Medical College Affiliated Jingzhou Hospital,Central China Technology University,Jingzhou,Hubei,China,434020;Progress in the Determination of Constituents Content of Common Floweringqince Fruit and Its Identification[J];Guiding Journal of Traditional Chinese Medicine and Pharmacy;2009-03
10 LI Li1,CHANG Bo2(1.P.E.Department of Jiaying University,Meizhou 514015,Guangdong China;2.Dept.of Human Sports Science,Shenyang Physical Education Institute,Shenyang 100102,Liaoning China);Research on the Preventive Effect of Aralia Elata on Oxidation Impairment of Renal Function Following Overtraining in Rats[J];Journal of Harbin Institute of Physical Education;2011-02
China Proceedings of conference Full-text Database 1 Hits
1 ;Study on the Method of Determination the Contents of ursolic acid and oleanolic acid in Prunella vulgaris L.and its triterpene effective Fraction[A];[C];2007
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 HU Jun ping,YANG Jian hua,DU Nian sheng College of Pharmacy,Xinjiang Medical University,Urumqi 830054,China;Determination of oleanolic acid in Vitis amurensis by TLCS[J];West China Journal of Pharmaceutical Sciences;2003-01
2 BAI Yu-jun 1, YANG Xiao-sheng 1* , KANG Wen-yi 1,HAO Xiao-jiang 1, XU Xiao-jie 2,SONG Bao-an 31.The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002,China; 2. College of Chemistry and Molecular Engineering,Peking University, Beijing 100871,China; 3. Research and Development Center of Fine Chemicals, Guizhou University, Guiyang 550025,China;The modificated compounds from ursolic acid and their antitumor activities[J];West China Journal of Pharmaceutical Sciences;2003-02
3 ZHOU Gen-Tu(Forestry Department of Anhui, Hefei 230001, China);High-yield Culture Technology of Chaenomeles speciosa[J];Economic Forest Researches;2003-04
4 DING Zhi-En~1,YIN Biao~1,ZHOU Gen-Tu~2,CHENG Liu~1(1.Anhui Agricultural University,Anhui 230036,Hefei,China;2.Forestry Department of Anhui Province,Hefei 230001,Anhui,China);Microwave-assisted Extraction of the Active Ingredients in Chinese Flowering Quince[J];Nonwood Forest Research;2006-04
5 JI Zhi-ping, SU Yin-quan, L Ping-hui, Zhang Cun-li(College of Forestry Sciences,Northwest A & F University, Yangling 712100, China);Studies on Chemical Constituents and Antimicrobial Activities of Persimmon Leaves[J];Chemistry and Industry of Forest Products;2006-04
6 XU Shan, DENG Xu-kun1, CHEN Bin1, XU Chang-feng*, CHEN Qi (School of basic medical sciences,NJMU, Nanjing 210029; 1Pharmacy college, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China);Determination of oleanolic acid and ursolic acid in verbena officinalis L. using RP-HPLC[J];Acta Universitatis Medicinalis Nanjing(Natural Science);2006-03
7 唐春红(渝洲大学包装与食品工程系,重庆 400033) 项昭保 任绍光 石轶松 叶志义(重庆大学农业生物工程研究中心,400044);木瓜中齐墩果酸的提取工艺研究[J];SCIENCE AND TECHNOLOGY OF FOOD INDUSTRY;2000-04
8 WANG Wen-Ping,ZHOU Wen-Mei(Chemistry and Biology Engineering College, Guizhou University of Technology, Guiyang 550003, China);Extraction and Quantitative Determination of Oleanolic Acid in the Chinese Quince[J];Food Science;2004-09
9 WHANG Fang-an1,2 , HUANG Yong3, ZHOU Guo-ping2, WANG Lin4(1.Huazhong Agriicutural University, Wuhan 430023, China;2.Wuhan Polytechnic College, Wuhan 430023, China;3.Quality and Technology Supervision Bureau of Wuhan, Wuhan 430023, China;4.Guangdong Huixiang yuan Food Co. Ltd., Guangzhou 510665, China);Study on the Content of Oleanolic Acid in Chinese Flowering Quince Fruit Vinegar by HPLC[J];Food Science;2004-11
10 WANG Jia-xiang (College of Agri-forestry, Linyi Normal University, Shandong 276003,China);Analayses of Nutritive Composition and Processing Technology of the Fruit of Chaenomeles in Yizhou[J];Food Science;2005-08
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