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In Vitro Culture of Mammary Epithelial Cells in Goat

Gao Yajun, Tang Shuang, Sun Sujun, Zhang YongInstitute of Bio-Engineering, Northwest A & F University, Yangling, Shanxi 712100, China  
The mammary tissues from healthy xinong saanen goat during lactation have been cultured in vitro. The basic medium is D-MEM/F-12 and 10% fetal calf serum,and EGF(0.01mg/L),hydrocortisone (1mg/L),P(1mg/L), ITS(5mg/L),penicillin(0.05g/L) and streptomycin(0.05g/L) were added.The results showed that the goat mammary epithelial cell grows well and proliferates vigorousely.
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