Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Anhui Agricultural Science Bulletin》 2008-07
Add to Favorite Get Latest Update

Comparision of three methods of Genomic DNA Extraction from rubber tree

Huang Yan1 Guo Qingshui1 Xu Lixin1* Chen Shoucai2(1 College of Life science and Agriculture of Hainan University,Haikou 570228;2 Chinese Academy of Tropical Agriculture Science,Danzhou 571101)  
In order to get the genomic DNA of Hevea brasiliensis which is suitable for normal PCR reaction,Leaves of rubber tree were used to isolated the genomic DNA by CTAB method、SDS method and salting out method.The Genomic DNA samples extracted by three methods were tested by agarose gel elctrophresis and restriction endonucleas digestion,Based on the comparative analysis of yield and quality of the DNA samples by three methods,the result showed that DNA yield of CTAB method was higher than that of SDS method and salting out method.the rubber tree genomic DNA isolated by the CTAB methods can not only be digested susceptibly by restriction endonucleas,but also can meet a demand for study of subsequent molecular research such as PCR analysis and so on.
【Fund】: 农业部热带作物栽培生理学重点实验室开放课题基金“橡胶死皮关键基因的克隆与功能鉴定”(KLOF0502)
【CateGory Index】: S794.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 4 Hits
1 ZHONG Ganbin1,2) LI Weiguo1) ZHENG Xuexiang1,2) LI Hailin1,2) YAN Yuanyuan1,2) YI Xiaojie1,3) CHEN Zuxing1,2) (1 Rubber Research Institute, CATAS, Danzhou, Hainan 571737; 2 College of Agronomy, Hainan University, Danzhou, Hainan 571737; 3 Environment and Plant Protection Department of Hainan University, Danzhou, Hainan 571737);A Simple and Effective DNA Extraction Method for AFLP Analysis in Hevea brasiliensis Muell Arg.[J];Chinese Journal of Tropical Agriculture;2010-05
2 LI Ji,HUANG Tiandai,CAI Haibin,HUA Yuwei,HUANG Huasun Rubber Research Institute of Chinese Academy of Tropical Agricultural Sciences/State Key Laboratory Breeding Base of Cultivation & Physiology of Tropical Crops,Danzhou,Hainan 571737;Genetic Stability Analyses in Transfomants of Hevea brasiliensis[J];Chinese Journal of Tropical Crops;2013-04
3 LI Ji,HUANG Tiandai,CAI Haibin,HUA Yuwei,HUANG Huasun Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences / State Key Laboratory Breeding Base of Cultivation & Physiology of Tropical Crops,Danzhou,Hainan 571737,China;The PCR Analysis of Early Embryos in Hevea Somatic Embryogenesis Transformation[J];Chinese Journal of Tropical Crops;2013-05
4 YING Xiong-mei1,CAI Qing2,BI Yan1 LIU Xin-long1,MA Li1,MAO Jun1,LU Xin1(1.Sugarcane Research Institute,Yunnan Academy of Agricultural Sciences,Kaiyuan 661600,China;2.Biotechnology and Genetic Germplasm Institute,Yunnan Academy of Agricultural Sciences,Kunming 650231,China);Comparision between Two Sugarcane Genome DNA Extraction Methods[J];Sugar Crops of China;2009-04
【Citations】
Chinese Journal Full-text Database 1 Hits
1 AN Ze-Wei, HUANG Hua-Sun*State Center for Rubber Breeding, Key Laboratory of Tropical Crops Physiology of Ministry of Agriculture, Rubber ResearchInstitute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China;A Method for Genomic DNA Extraction from Leaves of Rubber Tree (Hevea brasiliensis Muell. Arg.)[J];Plant Physiology Communications;2005-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 JIANG Xian-rong,YANG Jie,ZHOU Yan-mao(Department of Biology,Hefei Teachers College,Hefei 230061,China);Advances in the Application of Transgenic Technology and the Safety of Transgenic Organisms[J];Journal of Hefei Teachers College;2008-03
2 ZHENG Zhao-ying1, SUN Xiao-lei2, LIU Shi-qi1, GAO Jun-feng2(1Horticulture College, Shandong Agricultural University, Taian 271018, China;2Vegetable Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China);Study on ethylene distribution in cucumber plant and its year-round dynamics[J];Shandong Agricultural Sciences;2003-04
3 LIANG Tai-bo~1, ZHAN Shu-xiang~(2), SHI Chun-yu~(1*), WANG Ru-juan~1,FANG Fu-cheng~(2)(1.Agronomy College,Shandong Agricultural University, Taian 271018,China;2.Soil and Fertilizer Institute,CAAS,Beijing 100081,China);Effect of Huolisu on yield and nitrate content of spinach[J];Shandong Agricultural Sciences;2005-01
4 YE Bao-xing,MAO Da-chao,LIU Xue-chun( Shangdong Agricultural university ,Taian,271018);Study on the correlation of root activity and photosynthetic rate in the later growth duration of super-wheat[J];Shandong Agricultural Sciences;2005-04
5 SUN Hui-min~1,YU Zhen-wen~(1*),YAN-hong~2,SHI Gui-ping~2(1.Key Laboratory of Wheat Cultivation Physiology & Genetic Improvement,Ministry of Agriculture,Shandong Agricultural University,Taian 271018;2.Agrotechnical Extension Center of Longkou City,Longkou 265701,China);Effect of Phosphorus Rate Applied on Yield,Quality and Phosphorus Utilization Ratio in Winter Wheat under Different Fertility Soil[J];Shandong Agricultural Sciences;2006-03
6 ZHANG Wen-lan,LI Ru-yu,LI Qun,DUAN Nai-bin (Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China);A Rapid DNA Extraction Procedure for Maize Purity Test[J];Shandong Agricultural Sciences;2007-05
7 XIAO Bei-lei1,2,LI De-quan1,LI De-quan1,HU Xiao-li1(1.State Key Laboratory of Crop Biology,College of Life Science,Shandong Agricultural University,Taian 271018,China; 2.Agronomy Department of Dezhou College,Dezhou 253023,China);Effects of Salt Stress on Membrane Lipid Peroxidation and Endogenous Protective Systems in the Leaf of Transgenic Tobacco with ZmPP2C2 and Wild Tobacco[J];Shandong Agricultural Sciences;2008-01
8 YU Wen-yan,LIU Shi-qi*,LIANG Qing-ling,HAO Shu-qin(College of Horticulture Science and Engineering,Shandong Agricultural University,State Key Laboratory of Crop Biology,Taian 271018,China);Comparison of Growth and Physiological Indices between Diploid and Tetraploid Garlic Plants[J];Shandong Agricultural Sciences;2008-02
9 FAN Jing1,2,LIU Qing-zhong2,ZHANG Jun-lin2,3,WANG Jin1(1.Faculty of Landscape Architecture,Southwest Forestry College,Kunming 650224,China;2.Shandong Institute of Pomology,Taian 271000,China;3.Department of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China);A Modified Method for Genomic DNA Extraction from Juglans regia[J];Shandong Agricultural Sciences;2008-06
10 MA Li(Department of Biology,Zaozhuang College,Zaozhuang 277160,China);Two Fast Methods for Total RNA Extraction from Root Tubers of Sweet Potato[J];Shandong Agricultural Sciences;2008-06
China Proceedings of conference Full-text Database 10 Hits
1 Li Xiao-liang~(1,2) Li Jian-jiao~1 L(u|¨) Bao-lei~1 Chen Xiao-min~2 Wang Xiao-yang~2 Zhan Guofei~1 (1 College of Urban Construction and Environmental Sciences,Anhui Science and Technology University,Fengyang 233100,China;2 College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095,China);Effects of Conjunctive Application of Fertilizer and Gypsum on the Biological Properties of Potting Corn in Coastal Saline Soil[A];[C];2011
2 FAN Rong-hui~(1,2,3) HUANG Min-ling~(1,2,3) ZHONG Huai-qin~(1,2,3) WU Jian-shen~(1,2,3) LIN Bing~(1,2,3) YE Xiu-xian~(1,2,3) LUO Yuan-hua~(1,2,3) (1 Institute of Crop Sciences,Fujian Academy of Agricultural Science,Fuzhou 350013; 2 Flowers Research Center,Fujian Academy of Agricultural Science,Fuzhou 350013; 3 Fujian Engineering Research Center for Characteristic Floriculture,Fuzhou 350013);Cloning and Expression Analysis of Key Enzyme Genes in Blue Flowers from Strelitzia reginae[A];[C];2011
3 LI Ting-ting~1 ZHANG Xiu-xin~3 LV Ying-min~2 (1 Institute of Agricultural Information Chinese Academy of Agricultural Science,Beijing 100081; 2 College of Landscape Architecture,Beijing Forestry University,Beijing 100083;3 Institute of Vegetables and Flowers,Chinese Academy of Agricultural Science,Beijing 100081);Media Formula Screening for Eco-organic Type Soilless Cultivation of Potted Paeonia lactiflora[A];[C];2011
4 KONG Pei-pei YANG Shu-hua JIA Rui-dong GE Hong (Institute of Vegetables and Flowers,Chinese Academy of Agriculture Sciences,Beijing 100081);Effects of Different Arbuscular Mycorrhizal Fungi(AMF)Strains on Some Physiological Indices of Rosa hybrida 'Carola'[A];[C];2011
5 XU Ying CHEN Yu CHEN Su-mei ZHANG Hai-ping CHEN Fa-di GUAN Zhi-yong JIANG Jia-fu (College of Horticulture,Nanjing Agricultural University,Nanjing 210095);The Effect of SOD and CAT Activity in Leaves of Chrysanthemum Under Cold Treatment[A];[C];2011
6 FANG Xiao-jun WANG Kui-ling LIU Qing-chao LIU Qing-hua (College of Horticulture and Landscape Architecture Qingdao Agricultural University,Qingdao 266109);Research on Shade Tolerance of Convallaria majalis L.[A];[C];2011
7 Li Yijie1,Yuan Baozhong1,Bie Zhilong2,Kang Yaohu3(1.College of Plant Science and Technology,Huazhong Agricultural University;2.College of Horticulture and Forestry,Huazhong Agricultural University,Wuhan 430070,China;3.Institute of Geographical Sciences and Natural Resource Research,Chinese Academy of Sciences,Beijing 100101,China);Effects of drip irrigation threshold during vegetative growth stage on growth and yield of muskmelon inside plastic greenhouse[A];Chinese Society of Agricultural Engineering[C];2011
8 XIA Hui-long,CHENG Wen-wei,CHI Xiao-ya (College of Environmental Science & Engineering,Zhejiang Gongshang University,Hangzhou 310012,China);Effects of Cd stress on the growth and physiological properties of sugarcane[A];[C];2008
9 PENG Wentao,WANG Feng,CUI Zhongli,CAO Hui (Key Laboratory of Microbiological Engineering of Agricultural Environment,MOA, Nanjing Agricultural University,Nanjing,210095);The impacts of transgenic cotton on soil microbes' kanamycin resistance[A];[C];2009
10 YANG Sheng,ZHANG Huaxin,LIU Tao (Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China);Effect of Growth and Physiological and Biochemical of Plants under Salt Stress[A];[C];2011
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YING Yi-yi et al(Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101);Comparison on Several Extraction Methods of Genomic DNA from Hevea brasiliensis[J];Journal of Anhui Agricultural Sciences;2010-17
2 PENG Jian ying 1, SHU Huai rui 2, PENG Shi qi 1 (1 Research Center of Chinese Jujube, Agricultural University of Hebei, Baoding 071001, China; 2 Department of Horticulture, Shandong Agricultural University, Tai′an 271018, China);A method for genomic DNA isolation of Chinese jujube and acid jujube[J];Journal of Agricultural University of Hebei;2000-04
3 Zhan Yaguang, Zeng Fansuo(Northeast Forestry University, Harbin 150040, P. R. China);A Method for DNA Extraction from Mature Birch Leaves Rich in Polysaccharide[J];Journal of Northeast Forestry University;2005-03
4 Jin Wanmei1,2* Pan Qinghua2 Yin Shuping2 Dong Jing2 Jiang Lijie2 Yang Li2 Chen Qinghua2 Zhao Jianbo2 1 College of Agricultural and Biotechnology Science, China Agricultural Universi ty, Beijing, 100094; 2 Institute of Forestry and Pomology, Beijing Academy of Ag riculture and Forestry Science, Beijing 100093;Progress of the Genetic Stability and Breeding Behavior of Foreign Gene in Genetically Modified Plants[J];Molecular Plant Breeding;2005-06
5 Wang Lan 1,2 Long Yunming 2 Liu Yaoguang 2 1 Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, 510642; 2 College of Life Sciences, South China Agricultural University, Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Province, Guangzhou, 510642;A Method for Rapid Preparation of Plant Genomic DNA for PCR Analysis[J];Molecular Plant Breeding;2009-02
6 Xiong Guangming, Liang Guolu, Yan Yong, Xiang Suqiong, Wu Qing, Li Xiqing,and Jiang Dong(Department of Horticulture , Southwest Agricultural University and Laboratory of Agricultural Comprehensive Development,Chongqing 400716 China; Citrus Research I;DNA Extraction Method for AFLP Analysis in Citrus[J];Journal of Fruit Science;2002-04
7 ZHONG Luo-bao, CHEN Gu (College of Light industry and Food science, South China University of Technology, Guangzhou 510640, China);Study of a Simplified DNA Extraction Method for the Large-scale PCR Detection of Genetically Modified Crops[J];Modern Food Science and Technology;2008-08
8 WU Guan-ting~(1,2) HU Zhang-hua~2 LANG Chun-xiu~2 CHEN Xiao-yun~2WANG Fu-lin~2 JIN Wei~2 CHEN Jin-qing~(2) XIA Ying-wu~1(1. Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, Zhejiang 310029;2. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021);ESTABLISHMENT OF Agrobacterium-MEDIATED TRANSFORMATION SYSTEM FOR TALL FESCUE[J];Acta Agriculturae Nucleatae Sinica;2005-05
9 LUO Zhi yong, ZHOU Gang, CHEN Xiang hui, et al (Molecular Biology Research Center, Xiangya Medical College, Central South University, Changsha 410078, China);Isolation of high-quality genomic DNA from plants[J];Bulletin of Hunan Medical University;2001-02
10 YU Ya1,LIU Chuanliang2,MA Zhiying1,LI Fuguang2*(1. College of Agriculture, AUH,Baoding, Hebei 071001,China;2. Cotton Research Institute, CAAS,Anyang, Henan 455112, China);The Culture of Cotton Apical Meristem and its Application in Particle Bombardment Transformation[J];Acta Gossypii Sinica;2003-05
【Secondary References】
Chinese Journal Full-text Database 9 Hits
1 HUANG Jing-li 1 ,NIU Jun-qi 1 ,ZHU Hui 1 ,YANG Li-tao 1,2 ,LI Yang-rui 1,2 ,WANG Ai-qin 1* ( 1 Agricultural College,Guangxi University/State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,Nanning 530005,China; 2 Sugarcane Research Center,Chinese Academy of Agricultural Sciences/Sugarcane Research Institute,Guangxi Academy of Agricultural Sciences,Guangxi Key Laboratory of Sugarcane Biotechnology and Genetic Improvement,Ministry of Agriculture/Guangxi Key Laboratory of Sugarcane Genetic Improvement, Nanning 530007,China);Cloning and sequence analysis of promoter in sugarcane ethylene receptor gene(Sc-ERS)[J];Journal of Southern Agriculture;2013-05
2 LI Hailin1,2,LI Weiguo1,CHEN ZuXing1,2,ZHAO JianWen1,2,ZHAI QiLin1,2 1 College of Agronomy,Hainan University,Danzhou,Hainan 571737,China 2 State Center for Rubber Breeding,Insitute of Rubber,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China;Optimization and Establishment of MSAP Reaction System for Hevea brasiliensis[J];Chinese Journal of Tropical Crops;2011-04
3 ZHONG Ganbin1,2,LI Weiguo1,WU Chuntai1,YI Xiaojie3 1 Rubber Cultivation Research Institute,CATAS,Danzhou,Hainan 571737,China 2 Zigong Higher Technical School,Zigong,Sichuan 643000,China 3 Vocational Technical School of Zigong,Zigong,Sichuan 643000,China;AFLP Analysis of 188 Germplasm Material for Rubber Tree(Hevea brasiliensis)[J];Chinese Journal of Tropical Crops;2013-01
4 ZHAO Jianwen1,2,ZHANG Xiaofei2,ZHAI Qilin1,2,LI Weiguo2 1 College of Agriculture,Hainan University,Haikou,Hainan 570228,China 2 State Center for Rubber Breeding,Rubber Reseach Institute,CATAS,Danzhou,Hainan 571737,China;Contribution of Foreign Germplasms Used as Parents for the Cross-breeding of Hevea brasiliensis in China by EST-SSR Markers[J];Chinese Journal of Tropical Crops;2013-02
5 LI Ji,HUANG Tiandai,CAI Haibin,HUA Yuwei,HUANG Huasun Rubber Research Institute of Chinese Academy of Tropical Agricultural Sciences/State Key Laboratory Breeding Base of Cultivation & Physiology of Tropical Crops,Danzhou,Hainan 571737;Genetic Stability Analyses in Transfomants of Hevea brasiliensis[J];Chinese Journal of Tropical Crops;2013-04
6 LI Ji,HUANG Tiandai,CAI Haibin,HUA Yuwei,HUANG Huasun Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences / State Key Laboratory Breeding Base of Cultivation & Physiology of Tropical Crops,Danzhou,Hainan 571737,China;The PCR Analysis of Early Embryos in Hevea Somatic Embryogenesis Transformation[J];Chinese Journal of Tropical Crops;2013-05
7 NI Shu-bang,MAO Chang-li,WU Yu Yunnan Institute of Tropical Crops,Jinghong Yannan 666100,China;Genetic Diversity in Some Germplasm Resources of Hevea brasiliensis Determined by Fluorescent-AFLP Markers[J];Journal of Southwest University(Natural Science Edition);2012-02
8 LU Xin1,MAO Jun1,LIU Hong-bo1,LIU Xin-long1,MA Li1,SU Huo-sheng1,CAI Qing1,2,3(1Yunnan Key Laboratory of Sugarcane Genetic Improvement / Sugarcane Research Institute,Yunnan Academy of Agricultural Sciences,Kaiyuan 661600;2Biotechnology & Genetic Resources Institute,Yunnan Academy of Agriculture Sciences,Kunming 650223;3School of Life Sciences,Yunnan University,Kunming 650091);Innovation Germplasm of Erianthus rockii Ⅰ. Establishment of F_1 Intergeneric Distant Hybridization of Saccharumofficinarum L. and Erianthus rockii and the Identification of F_1 by SSR Molecular Markers[J];Journal of Plant Genetic Resources;2012-02
9 CHEN Ming-Hui , ZHANG Bao-Qing , SONG Xiu-Peng , CHEN Hu , YANG Li-Tao , LI Yang-Rui , and CHEN Bao-Shan Guangxi University / State Key Laboratory of Subtropical Bioresources Conservation and Utilization, Nanning 530005, China; Sugarcane Research Center, Chinese Academy of Agricultural Sciences / Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences / Guangxi Crop Genetic Improvement and Biotechnology Laboratory / Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture / Guangxi Key Laboratory of Sugarcane Genetic Improvement, Nanning 530007, China; Guangxi Forestry Research Institute, Nanning 530002, China;cDNA-SCoT Analysis of Differentially Expressed Genes in Sugarcane Induced by Leifsonia xyli subsp. xyli[J];Acta Agronomica Sinica;2013-06
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Shi Yonggang Zheng Yonglian Li Jiansheng Liu Jilin(State Key Lab. of Crop Genetic Improvement, Wuhan, 430070);Mapping CMS-S Restores Gene Rf_3 with RFLPs and RAPDs[J];Acta Agronomica Sinica;1997-01
2 Fu Junhua Li Lancheng Liu Xinzhi Peng Zebin Huang Changling(Institute of Crop Breeding and Cultivation, CAAS, Beijing, 100081);Studies on the Protocol Optimum and Preliminary Applification RAPD in Maize[J];Acta Agronomica Sinica;1997-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved