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《Anhui Agricultural Science Bulletin》 2008-11
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Optimization of the Electrotransformation in Sinorhizobium Meliloti

Wang Beiyan et al.  
In order to investigate the electrotransformation condition of Sinorhizobium meliloti variable curve was devised by different cell cycles time,resuscitative buffer,electrotransfection buffer,pulsed electric field and resistance.The experiment results indicate that logarithmic growth phase,resuscitative buffer Ⅴ,electrotransfection buffer Ⅳ,23kv/cm and 200 resistance are regarded the best electrotransfection condition of Sinorhizobium meliloti.
【CateGory Index】: S541.9
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