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《Anhui Agricultural Science Bulletin》 2009-03
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Apoptosis Induction of Blood Cells in Aristichthys nobilis by Cu~(2+) Stress

Yuan Lu et al.(School of Life Science,Southwest University;Key Laboratory of Eco-Environments in Three Gorges Reservoir Region(Ministry of Education);Key Laboratory of Aquatic Science of Chongqing;Chongqing 400715)  
Effects of Cupric ion(Cu2+) at different concentrations on apoptosis induction in cultured blood cells of Aristichthys nobilis were studied by AO and EB staining for fluorescence observation and Swiss Giemsa staining for optical microscopy observation.The results of morphological characteristics and apoptosis rate analysis showed that Cu2+ stressed the Aristichthys nobilis blood cells and caused characteristic features of apoptosis,such as rounding of cells and nuclei,condensation and peripherization of nuclear chromatin,and formation of apoptotic bodies.Rates of apoptosis were elevated with the increase in Cu2+ concentration and treatment time.All of the results together indicated that apoptosis could be induced in Aristichthys nobilis blood cells by Cu2+ in a dose-and time-dependent manner.
【Fund】: 三峡库区生态环境教育部重点实验室开放基金资助
【CateGory Index】: S965.113
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