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《JOURNAL OF ANHUI AGRICULTURAL SCIENCES》 1999-05
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Genetic Diversity Detected with RAPD Markers Among Rice Cultivars(Oryza sativa L.) and Its Utilizaton in Hybrid Rice Breeding

Zhang Peijiang et el(Anhui Academy of Agricultural Sciences,Key Laboratory of Rice Genetics & Breeding,Ministry of Agricultural,Hefei 230031)  
Seventeen parents of hybrid rice and 3 new plant type lines and 24 Nuda,Javanica cultivars were used to evaluate genetic diversities by RPAD markers.It was explored that the possibility of genetic diversity estimated from RAPD banding patterns that could be used in hybrid rice breeding.Twenty-two 12-mer random primers were used for PCR reactions.The total of 98 distinguishable PCR products were detected by agarose gel electrophoresis among 44 parents,17 random primers were polymorphic and 47.9% of total products were different at least between two parents.Each of those amplified bands was taken as one variance which was record as 1 for present and 0 for absent.Based on the pair-wise comparsons of amplification products the genetic distance was calculated using Nei's genetic distance and a dendrogram was constructed using an unweighted pair group method with arithmetical averages (UPGMA).The indica and japonica cultivars were easily classified into separate groups by cluster analysis,and temperate and tropical japonica cultivars were classified into separate subgroups,too.But Nuda and javanica cultivars were usually clustered in mixed subgroup.The results from the dendrogram were found that the economical heterosis between temperate japonica cultivars was low,the heterosis between subgroups was stronger and the heterosis between groups (indica/japonica) was strongest.Making use of Nuda and javanica,parents of hybrid rice with distinct ecotype were bred,and super highyield hybrid rice combinations had been bred.
【Fund】: 农业部“八五”应用基础研究项目;安徽省农科院青年基金
【CateGory Index】: S511.032
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