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《Journal of Anhui Agricultural Sciences》 1999-06
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Study on the Method of Mutant Induced by EMS Treating Rice Somatic Callus

Zhang Xiaolin et al (Zhejiang Wenzhou Agricultural Institute, Wenzhou 325006)  
The method and optimum concentration that EMS was used to treat rice somatic callus for mutant induction and some mutant lines were obtained. The result showed that the concentration of 1~5m g/L 50%LD of EMS was used to soak rice somatic calli for two hours; and in dropping method, the concentration was 1~5m g/L but 0.1ml EMS solution was added on the surface of callus every tube. According to the result of mutation of generation the concentration of 1m g/L was appropriate. The mutation ratio of generation was significantly higher than check after EMS treatment.
【CateGory Index】: S511.035
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