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《Journal of Anhui Agricultural Sciences》 2003-04
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The Potential Herbicide-resistant Gene Flow of Transgenic Glyphosate-resistant and Glufosinate-resistant Oilseed Rapes to Orychophragmus Violaceus

Song Xiaoling et al (Weed Research Laboratory, Nanjing Agricultural University , Nanjing,Jiangsu 210095)  
The potential herbicide-resistant gene flow of glyphosate-resistant and glufosinate-resistant transgenic rape to Orychophragmus violaceus were studied under pollination. The germination and growth of male pollen grain on female stigmas were observed with fluorescence microscope. The results showed pollen grain germination and growth of two varieties of transgenic oilseed rapes on the female stigmas was similar. Compared with self-pollination of O. violaceus, the adhesion number of pollen grains decreased significantly and adhesion line delayed; pollen grains couldn't germinated normally with abnormal pollen tubes. Pollen couldn't penetrate papilla cell, enter styles and embryo sacs. The observing results demonstrated the sexual compatibility between them was very low. The fact that emasculated O. violaceus pollinated with the males pollen grains could not germinate, further proved the incompatibility between them. All results showed the potential gene flow between them were very low.
【Fund】: 国家科技部转基因专项基金E2 0 0 1 0 2资助
【CateGory Index】: S565.4
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