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《Journal of Anhui Agricultural Sciences》 2003-06
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Study on Liquid Fermentation of Rhizopus delemar Lipase and Enzyme Properties

Liu Bin et al (Department of Biotechnological and Sciences Hefei University, Hefei 230022)  
The liquid fermentation conditions of Rhizopus delemar lipase were studied. The results suggested that the optimal medium be 5?g wheat bran,5?g soybean cake,0.3?g sucrose,and 0.3?g NaNO 3.The best culture condition was the inoculum of 28?℃ and at temperature of 28?℃ were 2?ml(spore concentration 10 7/ml),respectively.The lipase properties of Rhizopus delemar were also studied. The optimum temperature and optimum pH were 35?℃ and 6.5, respectively.The semiactive temperature was 47.5?℃, lipase was relatively stable at pH range of 6~8 after incubated in different pH solution at room temperature.
【Fund】: 安徽省自然科学基金项目 (0 1 0 4 31 0 2 );; 安徽省优秀青年科技基金资助
【CateGory Index】: Q556
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