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《Journal of Anhui Agricultural Sciences》 2004-04
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Effect of Osmotic Stress on Proline and Endogenous Hormone Content in Flue-cured Tobacco Leaves under Different Nitrogen Forms

Wang Yaofu et al (College of Agronomy, Henan Agricultural University, Zhengzhou,Henan 450002)  
The method of enzyme-linked immunosorbant assay was employed to determine the effect of osmotic stress on content of endogenous hormones in flue-cured tobacco leaves under different nitrogen forms. The results showed that the content of PRO, abscisic acid (ABA) and gibberellin (GA 3) in the leaves was higher and the content of heteroauxin (IAA) was lower when tobacco was fertilized with ammonium (NH 4 +-N) than that of nitrate (NO 3 --N) without osmotic stress, and that the content of ribosylzeatin (ZR) was increased when tobacco was fertilized with ammonium + nitrate (NO 3 --N + NH 4 +-N). It was observed that all the nitrogen forms could significantly increase the content of PRO in the leaves under osmotic stress when the osmotic potential was at -0.3?MPa.At the same time, the content of ABA tended to raise, and the content of IAA,GA 3 and ZR obviously decreased respectively when tobacco fertilized with both NH 4 +-N and NO 3 --N,while the content of all the endogenous hormones determined were increased in varying degrees when tobacco was fertilized with NO - 3-N + NH 4 +-N. With the increasing of osmotic stress, it was found that NH 4 +-N could significantly decline the content of PRO, and increase the content of GA 3 and ZR, but had not significant effect on the changes of ABA and IAA, and NO 3 --N did not significantly affect the changes of endogenous hormones in tobacco leaves though the content of PRO could be increased continuously. It was also found that the content of PRO, ABA,GA 3 and ZR could be decreased by NO 3 --N + NH 4 +-N, but no significant change on IAA was observed. These results indicated that GA 3 might be the main signal substance in tobacco plant under osmotic stress when tobacco was fertilized with NH 4 +-N.
【Fund】: 国家烟草专卖局资助项目 (1 1 0 2 0 0 30 2 0 0 9)
【CateGory Index】: S572
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