Studies on the Enzyme Hydrolysis of Soluble Polysaccharide from Momordica Charantia
WU Jia-di et al (Biological Technology College of Shenyang Agriculture University,Shenyang,Liaoning 110161)
The soluble polysaccharide of Momordica Charantia was extracted, then separated by ethanol. After removing the impurity such as the pigment and protein, the polysaccharide was further purified with DEAE-32 column chromatography until the single peak was got, which was named PMCⅠ.Determining its physical and chemical character showed that PMCⅠ belonged to polysaccharide. Then cellulase and neutral protase respectively were added in the extraction and purification of PMCⅠ,the most appropriate condition of PMCⅠ hydrolysis can be got by variance tests. The most appropriate condition of cellulase was: the temperature of extraction,50℃;pH, 6.0 and quantity of enzyme,5%.That of neutral protase is: the time, 48 hours; the pH, 7.0,and the quantity of enzyme 10%. Comparaing ethanol precipitation with enzymatic treatment showed that the latter not only make the technological conditions mild, but also improve the productive rate and content of PMCⅠ remarkably.