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《Journal of Anhui Agricultural Sciences》 2005-02
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Similarity Comparison and Evolution of Soybean and Cotton RGA

ZHU Mei-Xia et al (Hebei University of Engineering,Handan,Hebei 056038)  
There are conserved domain in protein products of the many known gene, such as Nucleotide Binding Site (Nucleotide Binding Site, NBS), LRR(leucine rich repeat, LRR) and TM(transfer membrane,TM).In this study, the degeneration primer was designed based on the conserved NBS of the genes of N,L6,and RPS2.Specific fragments of 517bp were amplified from genomic DNA of Soybean. The 16 fragments were obtained and they were sequenced. Moreover, They were compared with cotton RGAs and cloned soybean RGAs by DNA man software. The results showed the cloned RGAs in this experiment had high similarity to the known soybean RGAs. But it low similarity to cotton RGA were obtained by same primers. The results revealed molecular mechanism for evolution of soybean RGA and cotton RGA.
【Fund】: 国家 8 63计划 (2 0 0 1AA1 1 1 0 1 );; 国家转基因植物专项 (J99 A 0 0 9);; 海南省重点科技项目 (0 1 2 0 2 )资助
【CateGory Index】: S565.1;S562
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