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《Journal of Anhui Agricultural Sciences》 2005-06
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Methods of Purification of Cellulase and Preparation of Protoplasts

ZUO Hong-mei et al (College of Biology,Shenyang Agricultural University,Liaoning Province Key Laboratory of Biotechnology, Shenyang, Liaoning 110161 )  
Trichoderma viride was used as the material in this experiment,cellulase was produced by solid state fermentation with the straw powder and crude cellulase was gotten by immertion,then by three different treatments -(NH4)2SO4 classified precipitate,ultrafilter and Sephadex G-75 chromatography. Three different treatments were done separately for the preparation of protoplasts of the young tomato leaves and the results were that the high purified cellulase got less protoplasts and cellulase by the ultrafilter had the best effect.
【CateGory Index】: TQ925
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