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《Journal of Anhui Agricultural Sciences》 2005-07
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Optimal of RAPD Marker in Dactylis glomerata

ZENG Bing et al (Department of Grassland Science, Sichuan Agricultural University, Yaan, Sichuan 625014)  
Based on the genomic DNA extracted from Dactylis glomerata,some essential factors that might affect the result of RAPD assay were tested and compared.An optimal reaction system suitable for the assay and usage of RAPD in Dactylis glomerata was established,which was 2.0?mm/L Mg~(2+),200?μmol/l DNTP,1.0U Taq DNA polymerase,0.5pmol/μl primer, 60 ng template DNA in a volume of 20μl amplification reactions system.
【Fund】: 国家自然基金项目(30371022)资助。
【CateGory Index】: S543.3
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