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Construction of Plant Transformation Vector of FaDREB1 Gene for RNAi

LIU Ke-jie et al (Shenyang Agricultural University, Shenyang,Liaoning 110161)  
A plant transformation vector was constructed in this experiment.Plasmid pART27-FaDREB1(1)-FaDREB1(2) were derived from pMD18-T-FaDREB1,including FaDREB1 gene controlled by CaMV 35s promoter. Then the recombination plasmid was introduced into agrobacterium LB4404 by direct entering ways.
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