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《Journal of Anhui Agricultural Sciences》 2005-10
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Determination of the Chlorogenic Acid in Fermented Liquor of Eucommia ulmoides Oliv Leaf with RP-HPLC

LIU Chuan-an et al (School of Science, Yichun University, Yichun,Jiangxi 336000)  
A determination method of chlorogenic acid by high performance liquid chromtograph and photodiode array detector was established. The chromatographic column, Symmetry Sheild RP18 (3.9 mm×300 mm, 5 μm), water-methanol-acetic acid (88∶12∶1.2, V/V) mobile phase with 1.0 ml/min flow rate, the detected wavelength (326 nm), and the column temperature(25 ℃) were adopted. The calibration curve of chlorogenic acid was linear under the content of 2.24~11.2 μg, the correlation coefficient was 0.999 3. The method was simple, accurate and had wider linear range. The method can be adapted to control the quality of material and products that contained chlorogenic acid.
【Fund】: 江西省教育厅重点资助项目。
【CateGory Index】: TQ464;
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