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《Journal of Anhui Agricultural Sciences》 2005-12
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High Efficient System of Plant Regeneration for Genetic Transformation of Lilium

LI Hong-yu et al(College of Horticulture,Shenyang Agricultural University,Shenyang,Liaoning 110161)  
A genetic transformation system of plant regeneration from aseptic leaves induced by bulb was developed for the Oriental hybrid lily,Lilium Sorbone and Lilium Siberia.The excised leaves from aseptic seedlings produced shoots with 100 % regeneration rate on a MS basal medium with a supplement of 2 mg/L BA and 1 mg/L ZT.The shoots were able to form roots on the MS basal medium supplemented with 0.75 mg/L NAA.The regenerated shoots were sensitive to G418 and PPT,the concentration of G418 and PPT for screening genetic transformation plantlets were 80 mg/L and 1.75 mg/L,respectively.However,the shoots were tolerant to Carbenicillin,the concentration of which ranging from 300 mg/L to 400 mg/L was suitable for screening.
【Fund】: 江苏省高技术研究项目(BG2003307)
【CateGory Index】: S682.29
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