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《Journal of Anhui Agricultural Sciences》 2005-12
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Study on Protoplast Formation Condition of Trichoderma

LIN Ying et al(College of Life Science,Shenyang Agricultural University,Shenyang,Liaoning 110161)  
The protoplast formation condition of Trichoderma was studied.The result showed that the best protoplast formation condition was: the mycelia age was 18~20 h,the best osmotic stablizer system was 0.6 mol/L NaCL,phosphate buffer,Lywallzyme: Snailase=3∶3(mg/ml),32 ℃ and 3 h.The production of protoplast was perfect.
【CateGory Index】: Q932
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