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《Journal of Anhui Agricultural Sciences》 2006-06
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Study on the Method of Actinomycetes Isolation from Soil

ZHENG Ya-nan et al(Shenyang Agricultural Uniuersity,Shenyang,Liaoning 110161)  
The optimum concentration of the inhibitor potassium dichromate(K2Cr2O7) in the plate medium was studied;meanwhile,the study on pretreatment of soil samples collected from corn field was conducted.The results showed that the suitable method of the selective isolation of actinomycetes was: after the collected soil suspension(10 %) added with 6 % yeast extract and 0.05 % SDS(5 mmol/L phosphate buffer)was stirred at 40 ℃,pH 7 for 20 minutes,or the soil suspension(10 %) added with 6 % peptone and 0.05 % SDS(5 mmol/L phosphate buffer) was stirred at 50 ℃,pH 7 for 10 minutes,then the soil suspension diluted with water to 1 % was inoculated with the method of plate dilution on Gause's starch agar added with 150 mg/L K2Cr2O7 medium for 7 days and these pretreatments were effective on actinomycetes' isolation.
【CateGory Index】: S154.3
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