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《Journal of Anhui Agricultural Sciences》 2006-15
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Optimization of SRAP System in Maize

ZHAO Xin-liang et al (Henan Institute of Science and Technology,Xinxiang,Henan 453003)  
Sequence-related amplified Polymorphism(SRAP) is a molecular marker technology newly developed by Li and Quiros,which has the characteristics of simplicity,reliability,moderate throughput ratio and distributed evenly in genome.It has been widely applied in the fields of genetic map construction,plant comparative genomics,gene mapping and cloning,cDNA fingerprinting and genetic diversity.In order to adopt SRAP approach in the study on maize germplasm and heterosis,optimizing experiments for the SRAP PCR reactions including PCR Protocol,concentrations of DNA template and Mg~(2+) were carried out.The results showed that the most suitable Protocol was initially denaturing at 94℃ for 5 min;then 94 ℃,1 min;35 ℃,l min and 72 ℃,1 min for the first five cycles,then the annealing temperature was raised to 50 ℃ for another 35 cycles.The optimum concentrations of DNA template and Mg~(2+) were 20 ng DNA/25 μ1 and 2.0 mmol/L Mg~(2+) in this SRAP-PCR system.The new establishment of SRAP system of maize was fully reproducible and with good stability.
【CateGory Index】: S513
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