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Preliminary Study on Antimicrobial Activity of Fermentation Broths of Oudemansiella mucida

DENG Xu et al (The National Base of Life Science and Biotechnology Education,Northwest A & F University,Yangling,Shaanxi 712100)  
Oscillation culture was carried out in Oudemansiella mucida(Schrad ex Fr).The obtained fermentation broths were extracted with EtOAC,n-BuOH in succession to give three sections: extract of EtOAC,n-BuOH and the remained fermentation broths.Their antimicrobial activities against five common phytopathologenic funguses were determined by filter paper method and solid culture medium method.The result indicated that the fermentation broths markedly inhibited five phytopathologenic funguses.The metabolites with antimicrobial activity were mainly congregated in EtOAC extract,with the minimal inhibiting concentration(MIC) for Alternaria brassicae,Alternaria longipes and Gloesporum fructigenum being 10 mg/ml,the MIC for Fusarium graminearum and Alternaria alternata being 5 mg/ml.
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