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Isolation and Culture of Single Oocyst of E.tenella

ZHANG Zhu-ming et al(Animal College of Anhui Agricultural University,Hefei,Anhui 230036)  
4 strains of E.tenella were isolated with single oocyst technique and agar-drop method.5 Lohmann-cocks with 5 day-age were inoculated with the oocyst.The infected rate was 60 %,40 %,20 % and 20 % and final rate was 35 % in total.
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