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《Journal of Anhui Agricultural Sciences》 2006-20
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Study on Submerged Fermentution Culture of Peanut Rhizobia

WANG Bao-lin et al(College of Life Science and Engineering,Huanggang Normal University,Huanggang,Hubei 438000)  
Eight kinds of culture media were designated to carrying on shaking culture,selecting the YMA,the YMA ammonium phosphate dibasic,the extraction of soil,the defatted soybean leaching solution four kinds of culture medium carried on submerged fermentation culture.The fermentation conditions were controled in 28 ℃,pH 7.0,the oxygen quantity dissolved was above 90 %,and cultured on 5 days,samples were taken,bacteria quantity were counted on time and growth curve were determined.The results indicated that the biggest biomass(hundred million/ml) of peanut Rhizobium in four kinds of culture medium was 12.3,15.8,30.2 and 53.2,respectively;The generation time(hour) of logarithmic phase was 9.112,9.053,9.086 and 8.993 respectively.The longer the logarithmic phase was,the bigger the biomass was.The main fermentation condition influence factor and the control method were discussed in the article.
【Fund】: 湖北省教育厅重点研究项目(2003A002)
【CateGory Index】: Q93-33
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