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《Journal of Anhui Agricultural Sciences》 2007-02
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Study on Method Improvement of Preparing Chitosan

CAO Wei-xing et al(College of Land and Resources,Shenyang Agricultural University,Shenyang,Liaoning 110161)  
In order to acquire high degree of deacetylation and high viscosity chitosan,the improved method for preparing chitosan from river crab hull in the laboratory was studied in this article.The degree of deacetylation and viscosity average weight of molecular were mensurated and the best condition was found through range analysis,which was taking off protein with 15 % NaOH,1h and 100 degree,taking off calcium with 15 % HCl,4.5 hours and 25 degree,taking off acetyl with 45 % NaOH,3 hours and 130 degree.The degree of deacetylation was more than 85 %,and the viscosity average weight of molecular was more than 700 000.The decoloring phase and degradation was left out.The color of product was gray or cuticolor.The quality of chitosan was ensured.Results showed that it was possible to prepare chitosan from the crab of Panjin.
【CateGory Index】: R318.08
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