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《Journal of Anhui Agricultural Sciences》 2007-04
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Determination of Amarogerth in Lomatogonium Rotatum by RP-HPLC

YU Qing-fen et al (Chemistry Department,Qinghai Institute of Nationalities,Xining,Qinghai 810007)  
In this article,RP-HPLC was applied to determine the content of amarogerth in Lomatogonium rotatum.The sample were separated on the column of Kromasil-C18(250 mm×4.6 mm,5 μm) which eluted with methanol and water(0.04% phosphoric acid).The ratio of methanol and water was 52∶48 with detected wavelength at 254 nm,flow rate at 0.8 ml/min,column temperature at 30 ℃.The result showed that the compound was mostly separated.The linear ranges of amarogerth was 0.028~0.165 μg(r=0.999 5).This method was rapid and accurate.
【CateGory Index】: S567.239
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