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《Journal of Anhui Agricultural Sciences》 2007-07
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Studies on Technique of Chromosome Squashing of Root-tip in Lilium lancifolium Thunb

RONG Li-ping et al (College of Horticulture,Shenyang Agricultural University,Shenyang,Liaoning 110161)  
The roots of L.lancifolium Thunb.were used to study the effects of the sampling time,the pretreatment chemical solution,hydrolysing time and staining on the chromosome squashing.The results showed that the optimal sampling time of roots was about 8:30~9:30 am.Among the three pretreatment of chemical solutions,0.1% colchicine solution got best result.First,0.1% colchicine solution was pretreated at 4 ℃ for 24 h,then the root-tips were fixed with Carnoy solution for 6 h,hydrolysed with 1 mol/L HCl for 8 min at 60 ℃ and stained with modified Carbol fuchsin solution.With this method a very good effect of chromosome squashing could be obtained.The observation indicated that the chromosome number of 3 populations of Lilium lancifolium was 2n=3x=36.
【CateGory Index】: S682.29
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