Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Anhui Agricultural Sciences》 2007-07
Add to Favorite Get Latest Update

Studies on Technique of Chromosome Squashing of Root-tip in Lilium lancifolium Thunb

RONG Li-ping et al (College of Horticulture,Shenyang Agricultural University,Shenyang,Liaoning 110161)  
The roots of L.lancifolium Thunb.were used to study the effects of the sampling time,the pretreatment chemical solution,hydrolysing time and staining on the chromosome squashing.The results showed that the optimal sampling time of roots was about 8:30~9:30 am.Among the three pretreatment of chemical solutions,0.1% colchicine solution got best result.First,0.1% colchicine solution was pretreated at 4 ℃ for 24 h,then the root-tips were fixed with Carnoy solution for 6 h,hydrolysed with 1 mol/L HCl for 8 min at 60 ℃ and stained with modified Carbol fuchsin solution.With this method a very good effect of chromosome squashing could be obtained.The observation indicated that the chromosome number of 3 populations of Lilium lancifolium was 2n=3x=36.
【CateGory Index】: S682.29
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 4 Hits
1 Zhong Yuquan, Li Haobing and Wang Kejuan Institute of Agrobiological Genetics and Physiology,Jiangsu Academy of Agricultural Sciences,Nanjing,210014 Institute of Vegetable Crops,Jiangsu Academy of Agricultural Sciences,Nanjing,210014;Analysis of Karyotype and C-banding Pattern of Zijinshan Lily[J];Jiangsu Journal of Agricultural Sciences;1993-03
2 Yang Li-ping 1 Liu Xue-mei 2 Zhang Xiao-fang 1(1.Northeast Forestry University, Harbin 150040) (2.Suihua Educational Academy, Suihua 152054);STUDY ON CYTOLOGY IN LILIUM LANCIFOLIUM THUNB .[J];;1997-01
3 Tolgor; Liu Libo(Department of horticulture, Jilin Agricultural University Changchun 130118);STUDIES OF KARYOTYPES OF 5 SPECIES IN LILIUM FROM JILIN[J];JOURNAL OF WUHAN BOTANICAL RESEARCH;1996-01
4 Li Weimin and Meng Xianshu (Shenyang College of Pharmacy, Shenyang 110015) Gao Ying and Wang Liqun (Inner Mongolia Huhhot Traditional Chinese Medicine Factory, Huhhot 010020);Karyotype Analysis on Bulbus Lilii[J];China Journal of Chinese Materia Medica;1991-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Pan Youzhao Ke Zuntao Zhao YuYing(Gardening and Horticulture College of Yangtze University,jingzhou hubei,434025);The Effects of Lilium davidii var. Unicolor of Tissue Culture Due to Different Explants[J];Anhui Agricultural Science Bulletin;2007-19
2 SU Jie-chao et al (College of Horticulture,Shenyang Agricultural University,Shenyang,Liaoning 110161);Study on the Conservation of L.concolor var.buschianum Baker[J];Journal of Anhui Agricultural Sciences;2007-09
3 MA Cheng-liang et al(Biological Engineer College,Weifang University,Weifang,Shandong 261061);Discussion on the New Records of Distribution of Plant Resource in Shandong Province[J];Journal of Anhui Agricultural Sciences;2008-06
4 MA Cheng-liang et al (Biological Engineering College,Weifang University,Weifang,Shandong 261061);Discussion on the New Records of the Distribution of the Seed Plants in Shandong Province[J];Journal of Anhui Agricultural Sciences;2008-12
5 XU Xiao-lin et al(School of Life Science,Xuzhou Normal University,Xuzhou,Jiangsu 221116);Observation on the Growth and Development Characteristics of Roots and Stems of Chlorophytum comosum[J];Journal of Anhui Agricultural Sciences;2008-14
6 WANG Hong-xia et al (Life Science and Technique Department Xinxiang Medical College,Xinxiang,Henan 453003);Study on Morphology Diversity in Lilium regale Wilson[J];Journal of Anhui Agricultural Sciences;2008-16
7 WANG Hong-Xia et al(Department of Life Sciences,Xinxiang Medical College,Xinxiang,Henan 453003);Rapid Propagation of Lilium nepalense D.Don[J];Journal of Anhui Agricultural Sciences;2008-17
8 CAI Zheng-wang et al (Department of Chemistry and Life Science,Ankang University,Ankang,Shaanxi 725000);Research Advance on Clematis Plants[J];Journal of Anhui Agricultural Sciences;2008-18
9 WANG Hong-xia et al (Department of Life Science and Technology,Xinxiang Medical College,Xinxiang,Henan 453003);Protoplast Isolation of Aloe vera L.[J];Journal of Anhui Agricultural Sciences;2008-19
10 YANG Wei-ru et al (College of Landscape Architecture,Beijing Forestry University,National Engineering Research Center for Floriculture,Beijing 100083);Advances in Investigation,Introduction and Domestication of Wild Germplasm Resources of Lilium in China[J];Journal of Anhui Agricultural Sciences;2008-19
China Proceedings of conference Full-text Database 5 Hits
1 LI Hai-li PAN Jian-bin XU Wen-jie DONG Li (College of Landscape Architecture,Beijing Forestry University,Beijing 100083);Study on Communities' Species Diversity of Hemerocallis lilioasphodelus in Xiaowutai Mountain[A];[C];2007
2 HU Feng-rong LIU Guang-xin XI Meng-li WU Zhu-hua SHI Ji-sen (The Key Laboratory of Forest Genetics and Biotechnology of Chinese State Administration of Education,Nanjing Forestry University,Nanjing 210037);Giemsa C-banding Compare between Lilium lancifolium Thunb.and Yixing Lily[A];[C];2008
3 Guo Jin-lin Pei Jin Wan De-guang (Chengdu university of TCM Chengdu city 610075);Studies on Molecular Identification of Caulis Clematidis Armandii[A];[C];2007
4 ZHAO Xi-Nin,WANG Wen-He,HAO Pei (Department of Landscape,Beijing Agricultural College,Beijing 102206,China);The Karyotypes Analysis of Six Varieties of Lily[A];[C];2010
5 YUAN Li-li,LIU Qing-lin (Department of Ornamental Horticulture and Landscape Architecture,China Agricultural University,Beijing 100193,China.);Conservation,Evaluation and Enhancement of Wild Lily Germplasm in China[A];[C];2010
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 FU Yu-lan et al (Anhui Agricultural University,Hefei,Anhui 230036);Study on the Micropropagation of Dendrobium nobile[J];Journal of Anhui Agricultural Sciences;2006-03
2 CHENG Xiao lei,GUO Jun cheng,HE Hai sheng,YE Da peng,WANG Xiao ting (College of Economics and Technology,University of Science and Technology of China,Hefei 230052);Study on Chromosome Technique and Karyotype of Tobacco[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;2001-04
3 LIU Hui-qing,ZHANG Ai-xiang,CHANG Mei-hua,LIU Qing,GONG Xue-cheng(Agricultural Department,Hebei North University,Zhangjiakou,Hebei 075131,China);Study on Stem Section Reproduction of Dendrobe nobile in Vitro[J];Northern Horticulture;2008-01
4 Yang Yuzhen; Sun Tianzhou; Sun Ting; Lei Cheng.College of Landscape Architecture, Beijing For. Univ., 100083, P. R. China.;Tissue culture and rapid propagation techniques for Cymbidium hybridum .[J];Journal of Beijing Forestry University;2002-02
5 LIU Dong-yun~1,SHI Bao-sheng~1,LI Yin-hua~2,NIE Qing-juan~1,ZHANG Yan-guang~1(1.College of Gardens and Tourism,Agricultural University of Hebei, Baoding 071000, China;2.Hebei Vocational College of Political Sciences and Law, Shijiazhuang 050061, China);The bulbs augmentation influenced by the carbon resources PP_(333) and GA_3 during the low lily tissue culture[J];Journal of Agricultural University of Hebei;2005-02
6 TAN Jianzhong~(1) CHENG Jianping~(2) SUN Bingyao~(3) ZHANG Guoying~(1) LI Xinmei~(1) (~1College of Urbanology,Soochow University,Suzhou 215123,China;~2Silkworm Eggs-producing Farm of Xuguan,Suzhou 215151,China;~3College of Life Science,Soochow University,Suzhou 215123,China);Studies on Physiological Effects of Exogenous Betaine on the Salt Tolerance and Their Mechanisms in Mulberry[J];Acta Sericologica Sinica;2005-04
7 XU Qi jiang 1,CHEN Dian 2,LI Guiying 2 (1 Department of Biology,Teachers College of Heilongjiang Nongken,Acheng City 150301; 2 Department of Horticulture,Northeast Agricultural University,Harbin 150030);Study on the variation of tillered-onoin chromosomes in vitro culture[J];Journal of Northeast Agricultural University;2001-04
8 Wang Lun-shan, Yang Han-min, Wang Ya-fu and Kung Wen-jing(Department of Biotogy, Lanzhou University)Jia Ting-yao(Institute of Biotogy, Gansu Academy, Lanzhou);SOMATIC EMBRYOGENESIS FROM TISSUE CULTURE OF Fritiilariae pallidiflorae AND CYTOHISTOLOGICAL OBSERVATION[J];Acta Botanica Boreali-occidentalia Sinica;1989-02
9 Wang Lihong;Wang Yafu;Yang Hanmin;Gong Lin;Huan Weidong, (Department of Biology, Lanzhou University, Lanzhou 730000);STUDY ON CHANGES OF CARBOHYDRATES CONTENT AND ACTIVITIES OF AMYLASE AND SUCRASE IN MORP HOGENESIS OF CULTURED FRITILLARIAE PALLIDIFLORAE[J];Acta Botanica Boreali-occidentalia Sinica;1991-03
10 Tian Zengrong;Xu Qi;Zhu Jianfeng; and Yu Ling(Plant Cenetic Engineering and Breeding Key Laboratory of Shaanxi Province,Northwestern Institute of Botany, Academia Sinica,Yangling Shaanxi 712100);THE STUDY OF CHROMOSOME VARIATIONS IN REGENERATED PLANTS CULTURED FROM NULLISOMIC WHEAT× RYE[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1997-03
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 Tang Yan-cheng;Liang Song-yun Institute of Botany, Academia Sinica, Beijing;A PRELIMINARY SURVEY AND COMMENTS ON FUTURE STUDIES OF CHINESE LILIACEAE[J];Bulletin of Botanical Research;1983-02
2 Sun Chi-shi;Shi Meng;Fu Pei-yun Department of Pharmaceutical Botany, Shenyang College of Pharmacy, Shenyang Institute of Applied Ecology, Academia Sinica Shenyang, Liaoning;LILIUM IN NORTH--EASTERN CHINA[J];Bulletin of Botanical Research;1989-03
3 Li Maoxue Department of Biology, Beijing Unfversity Chen Ruiyang Department of Biology, Nanhai University;A SUGGESTION ON THE STANDARDIZATION OF KARYOTYPE ANALYSIS IN PLANTS[J];Journal of Wuhan Botanical Research;1985-04
4 Tolgor; Liu Libo(Department of horticulture, Jilin Agricultural University Changchun 130118);STUDIES OF KARYOTYPES OF 5 SPECIES IN LILIUM FROM JILIN[J];JOURNAL OF WUHAN BOTANICAL RESEARCH;1996-01
5 Li Maoxue;A Cytogenetic Observation on Tri-ploid Paconia Suffruticosa[J];Hereditas(Beijing);1982-05
6 Huang jiming ( Shanghai Horticultural Institute );HYBRIDIZATION BETWEEN LILIUM SPP.(LILIUM REGALE WILS. X L.DAVIDII VAR. UNICOLOR COTTON)[J];Acta Horticulturae Sinica;1982-03
7 Li Weimin and Meng Xianshu (Shenyang College of Pharmacy, Shenyang 110015) Gao Ying and Wang Liqun (Inner Mongolia Huhhot Traditional Chinese Medicine Factory, Huhhot 010020);Karyotype Analysis on Bulbus Lilii[J];China Journal of Chinese Materia Medica;1991-05
8 Tolgor(Jinlm Agriculture University,Changchun 130118) Zhao yu-tang(Department of Biology, Northeast Normal University, Changchun 130024) Xu jie-mei (Department of Biology, Sichuan University, Chengdu 610064);A CHROMOSOMAL STUDY OF EIGHT SPECIES IN ALLIUM SECT.RHIZIRIDIUM G. DON IN CHINA[J];Acta Phytotaxonomica Sinica;1994-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved