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《Journal of Anhui Agricultural Sciences》 2007-09
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Cloning and Sequencing of Ribosomal RNA Gene ITS in Colletotrichum orbiculare

TANG Jian-hui et al(State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237)  
The internal transcribed spacer regions(ITS1 and ITS2) of the ribosomal RNA gene from Colletotrichum orbicular were amplified with the polymerase chain reaction through the universal primers and sequenced.The size of total length was 481 bp;5.8S was 153 bp and ITS2 was 165 bp.The number of A,T,G and C was 110,106,118 and 147 respectively and the GC content was 55.1%.Based on the four sequences and another 24 species of published sequences of Colletotrichum spp in GeneBank database,the ITS1 and ITS2 phylogenetic trees were produced with the soft of Mega3.The Colletotrichum spp was divided into 13 groups.Colletotrichum orbiculare and Colletotrichum lindemuthiana were formed in a single group both in ITS1 and ITS2.So,it provided the molecule proof to design specialties primers to distinguish Colletotrichum orbiculare and Colletotrichum lindemuthiana from other species with the sequence of ITS.
【Fund】: 上海市重大科技攻关项目(03DZ19316);; 上海市科技兴农重点攻关项目[农科攻字2002(第3-1-2号)]
【CateGory Index】: S432.4
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