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《Journal of Anhui Agricultural Sciences》 2007-12
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Analysis of Genetic Relationship of 17 Houttuynia cordate Germplasm Resources With ISSR Marker

PENG Shuai et al(Institute of Life Science and Biotechnology,Bioenergy and Biomaterial Research Center,Hunan University,Changsha,Hunan 410082)  
17 germplasm resources of Houttuynia cordate were used as materials to analyze their genome polymorphism with ISSR markers.12 primers screened out from 50 primers were used for ISSR amplification.A total of 112 bands were generated,of which 95 bands were polymorphic band(the percentage of polymorphic band,PPB=84.8%).The average number of DNA fragments produced by each primer was 9.3.Nei' similarity coefficients and genetic distances were calculated with POPGENE software and the dendrogram were constructed with UPGMA.17 Houttuynia cordate materials were divided into three major groups while the genetic distance was 0.20.The results indicated that there actually existed much genetic diversity at molecular level among the germplasm resources of Houttuynia cordate,and the genuieness of Houttuynia cordate was related with the environments in some way.The relationship between genuieness,genetic and environmental factors of Houttuynia cordate were discussed.
【Key Words】： Houttuyniae cordata Thunb ISSR Cluster analysis Genetic Relationship Genuineness
【Fund】： 湖南省自然科学基金资助项目(06JJ50059)
【CateGory Index】： Q943
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