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《Journal of Anhui Agricultural Sciences》 2007-13
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Synthesis and Identification of the Immune Antigens for Herbicide 2,4-D

YU Ji-cheng et al (College of Plant Protection,Shenyang Agricultural University,Shenyang,Liaoning 110161)  
In the lab experiment,through using active ester method and mixed anhydride method,the immunity antigens and coating antigens of herbicide 2,4-dichlorphenoxyacetic acid(2,4-D) was synthesized by bovine serum albumin(BSA) or ovalbumin(OVA) and identified by ultraviolet spectrophotometry and ELISA.The results showed that the ratio of 2,4-D∶BSA was 24∶1,and that of 2,4-D∶OVA was 17∶1,respectively.The anti-2,4-D polyclonal antibodies were obtained by using the artificial immune antigen(2,4-D-BSA) to immune in the New Zealand rabbits.The titer of anti-serum was from 1.28×104 to 2.56×104.
【Fund】: 科技部“十五”食品安全重大专项资助项目(2001BA804A17-09)
【CateGory Index】: S481.8
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