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Preliminary Study on the Isolation and Fungicidial Activity of Fructus forsythiae Endophyte

YANG Run-ya et al(College of Life Science,Ludong University,Yantai,Shandong 264025)  
19 endophytic fungi isolated from the stem and leaf of Fructus forsythiae with PDA medium were cultivated in liquid medium respectively.Moist-heat sterilization was used to dispose the fermentation broth and mycelia were extracted with acetone after desiccated and grounded.The antifungal activity of the fermentation broth and the acetone extracts of mycelia on several plant pathogenic fungi were tested by means of mycelium growth rate.The results showed that the fermentation broth of 1 strain(5.26%)had a certain degree fungicidial activity(the inhibition rate more than 50%) and the acetone extracts of mycelia of 13 strains(81.25 %)exhibited more than 50% fungicidial activity.The compound of fungicidial activity mostly consisted of mycelia.2 strains(J4 and J8) with wide antifungal spectrum were screened out.The fungistasis of J8 to nine kinds of pathogenic fungi and the fungistasis of J4 to six kinds of pathogenic fungi were rather strong.
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