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《Journal of Anhui Agricultural Sciences》 2007-18
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Study on the Relation between HI Antibody Level and the Resistance to Challenge with Virulent H5 Subtype Avian Influenza Virus for Muscovy Duck

ZHANG Ji-pei et al(College of Life Science,Foshan University,Foshan,Guangdong 528231)  
50-day-old Muscovy ducks were grouped according to the different antibody levels to AIV H5N1 inoculated with corresponding inactivated vaccine,and each group was intramuscularly challenged with virulent AIV isolated from duck.The clinical signs and gross pathogenic changes were recorded at different timetable after Muscovy duck challenged with AIV.The laryngotracheal and cloacal swabs as well as partial internal organs were inoculated in 11-day-old chicken embryos intra-allantoid cavity for AIV isolation.The results showed that antibody levels detected by HI test less than 5.0log2 could not resist the challenge of virulent AIV H5N1 subtype,and clinical signs and different degrees of gross pathogenic changes were observed,furthermore,the shedding viruses were verified.If antibody levels detected by HI test was between 3.0log2 and 5.0log2,the degrees of latent period for showing symptoms,the pathogenic signs were lessened,and the partial resistant capability was found.While the antibody levels detected by HI test were more than 6.0log2,no clinical signs,gross pathogenic changes and shedding virus were detected.So far 6.0log2 could be regarded as the critical titer for H5 subtype AIV.Muscovy duck could resist virulent AIV challenge well when their attained to more than 6.0log2 by HI test.
【Fund】: 广东省重大农业攻关项目(2004A2090103 2005A20901003)
【CateGory Index】: S858.32
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