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Tissue Culture in vitro of Holland Lily Sorbonne

CHEN Xue-lin et al(College of Life Science,Northwest Normal University,Lanzhou,Gansu 730070)  
Objective The optimum culture route in vitro for Holland lily variety Sorbonne were searched,which could improve propagation rate.Method The bulb,tip bud,stem section and leaf of Holland lily variety Sorbonne were selected for in vitro culture.Result Bulb was the best explants and the optimum multiple bud induction medium was MS +6-BA 2.0 mg/L + IAA 1.0 mg/L with induction rate of 96.7 %.The central section of bulb displayed big organogenesis potential.The optimum regeneration and rooting medium for lily was MS +BA 1.0 mg/L+ IAA 0.4 mg/L and MS +IAA 0.5 mg/L,respectively,with rooting time of about 25 d.Conclusion Technical support were provided for the studying of lily breeding.
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