Effect of Bt and PI Triploid Transgenic Chinese White Poplar On Detoxicification Enzyme of Gypsy Moth Larvae
LU Shao-wei et al(Beijing Forestry University,Beijing 100083.)
[Objective] The propose of study was to provide the theoretical basis for rational utilization of transgenic plant.[Method] The 4th～5th instar larvae of gypsy moth were fed by the leaves of triploid transgenic Chinese white poplar of transferring Bt gene and arrowhead proteinase inhibitor gene and the activities of several detoxification enzymes of gypsy moth were determined.[Result] The activity of intestine esterase in larvae was increased at first,arrived at the highest after fed with transgenic Chinese white poplar for 24 h and decreased after 48 h.The activities of glutathione-S-transferase(GST) rose all the time.The activities of intestine esterase in larvae after fed by the leaves of transgenic Chinese white poplar Pb1,Pb33 and Pb47 for 48 h were 62.9 %,70.7 % and 74.5 % of that of CK and the activities of GST in larvae after fed by the leaves of transgenic Chinese white poplar Pb1,Pb33 and Pb47 for 24 h were 25.7 %,27.7 % and 17.9 % lower than that of CK.[Conclusion] The detoxification enzymes system in the midgut of larvae was changed and further toxic reaction of Bt protein could take place,which may be one of the important mechanisms of poisoning the pests by Bt transgenic Chinese white poplar.