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《Journal of Anhui Agricultural Sciences》 2007-27
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Study on the Tissue Culture Technique of Ligusticum chuanxiong Hort.

JIANG Wei-dong et al(College of Life Sciences,Sichuan University,Chengdu,Sichuan 610064)  
The study aimed to explore different tissue culture conditions of L.chuanxiong and optimize its induction and differentiation medium.In tissue culture with the root,stem segments and leaf of L.chuanxiong as explants,a lot of plantlets were obtained and the corresponding plant regeneration system was established.The optimum medium for inducing callus with L.chuanxiong root was MS+6-BA 0.8 mg/L+ NAA 1.2 mg/L and that with L.chuanxiong stem segments and leaf was MS+6-BA 0.5 mg/L+NAA 1.5 mg/L.The optimum medium for differentiating adventitious buds from root callus was MS+6-BA 2.2 mg/L+IAA 0.3 mg/L and that form stem segments and leaf was MS+ KT 2.0 mg/L+IAA 0.5 mg/L.The callus,differentiating adventitious bud and rooting induction of root explants were 84%,86% and 87% respectively.The callus induction rates of stem segments and leaf were 92% and 96% and their differentiation rate and rooting rate of adventitious bud were 98% and 93%.The transplanting survival rate of obtained plantlets was 98% seedling after-training.The medium with optimizing hormone combination was put forward and the high-effective induction rate,differentiation rate and rooting rate were obtained.
【Fund】: 国家科技攻关计划“十五”攻关课题(2004BA721A31)
【CateGory Index】: S567.239
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