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《Journal of Anhui Agricultural Sciences》 2007-27
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Study on the Tissue Culture Technique of Ligusticum chuanxiong Hort.

JIANG Wei-dong et al(College of Life Sciences,Sichuan University,Chengdu,Sichuan 610064)  
The study aimed to explore different tissue culture conditions of L.chuanxiong and optimize its induction and differentiation medium.In tissue culture with the root,stem segments and leaf of L.chuanxiong as explants,a lot of plantlets were obtained and the corresponding plant regeneration system was established.The optimum medium for inducing callus with L.chuanxiong root was MS+6-BA 0.8 mg/L+ NAA 1.2 mg/L and that with L.chuanxiong stem segments and leaf was MS+6-BA 0.5 mg/L+NAA 1.5 mg/L.The optimum medium for differentiating adventitious buds from root callus was MS+6-BA 2.2 mg/L+IAA 0.3 mg/L and that form stem segments and leaf was MS+ KT 2.0 mg/L+IAA 0.5 mg/L.The callus,differentiating adventitious bud and rooting induction of root explants were 84%,86% and 87% respectively.The callus induction rates of stem segments and leaf were 92% and 96% and their differentiation rate and rooting rate of adventitious bud were 98% and 93%.The transplanting survival rate of obtained plantlets was 98% seedling after-training.The medium with optimizing hormone combination was put forward and the high-effective induction rate,differentiation rate and rooting rate were obtained.
【Fund】: 国家科技攻关计划“十五”攻关课题(2004BA721A31)
【CateGory Index】: S567.239
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【References】
Chinese Journal Full-text Database 1 Hits
1 ZHANG Shi-xian(Chengdu Vocational College of Agriculture Science and Technology,Chengdu,Sichuan 611130);Analysis of the Growth Dynamic on Different Organ of Rhizoma Chuanxiong[J];Journal of Anhui Agricultural Sciences;2008-17
【Co-citations】
Chinese Journal Full-text Database 6 Hits
1 GUO Jin-lin WAN De-guang TANG Yuan PEI Jin (Chengdu university of TCM Sichuan Chengdu 610075);Resource Distribution and Commercial Primary Investigation of Caulis Clematidis Armandii[J];Journal of Chengdu University of Traditional Chinese Medicine;2007-01
2 JIANG Xiao-han,FENG Yi-fan,LIANG Han-ming,GUO Xiao-ling,MENG Qing (Institute of Materia Medica,Guangdong College of Pharmacy,Guangzhou,Guangdong 510006,China);HPLC analysis of butylidenephthalide in Szechwan lovage rhizome from different habitats[J];Journal of Guangdong College of Pharmacy;2006-06
3 GAN Guang-biao1,2,DUAN Hong-ping1,YANG Zhi-ling2,YANG Xu2,SHU Xiao1,YU Hua-hui2(1 College of Resources and Environment,Yunnan Agricultural University,Kunming 650201,China;2 Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Fuyang,Zhejiang 311400,China);Resources investigation of Turmeric Rhizome(RhizomaCurcumae Curcuma zedoaria(Berg).Rosc).and its germplasm collection in Southwest China[J];Guangxi Agricultural Sciences;2009-03
4 YE Meng YAN Xiao-yu LUO Cheng-de ZHAO Min XIANG Li LIU Guang-yu(College of Forestry and Horticulture,Sichuan Agricultural University,Yaan,Sichuan 625014);BARKED REGENERATION MECHANISM OF Phellodendron chinense var.glabriusculum SCHNEID BY USING ~(14)C-TRACER METHOD[J];Journal of Nuclear Agricultural Sciences;2007-02
5 TANG Da-xuan1,LUO Pei2,CHEN Lei1,TAN Zheng-huai1,LIANG Yuan-yuan1(1.Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041,China;2.West China School of Pharmacy,Sichuan University,Chengdu 610041,China);Anthraquinone extractive from Rheum Palmatum L.induced diarrhea through increasing the activities of NOS in mice[J];West China Journal of Pharmaceutical Sciences;2008-03
6 MA Yu-ying1,GUO Ding-ding1,JIANG Gui-hua1,XIAO De-yin2,GAO Ying3,TANG Sheng-wu1,MA Ling4,L Qiang1,XIAO Miao1(1.Chengdu University of Traditional Chinese Medicine,Chengdu,Sichuan,610075 P.R.China;2.Sichuan Yinfa Resource Exploration Co.Ltd.,Suining,Sichuan,629000 P.R.China;3.Tianjin Hospital,Tianjin,300211 P.R.China;4.Chongqing Three Gorges Medical College,Wanzhou,Chongqing,404020 P.R.China);Investigation report on the germplasm resources of Angelica dahurica[J];West China Journal of Pharmaceutical Sciences;2009-05
【Co-references】
Chinese Journal Full-text Database 1 Hits
1 YI Gui-hua et al(College of Earth Science,Chengdu University of Technology,Chengdu,Sichuan 610059);Absorption and Accumulation Characteristics of the Rhizoma of Genuine Chinese Medicinal Material Ligusticum chuanxiong Hort.Produced in Sichuan Province to Heavy Metals in Soil[J];Journal of Anhui Agricultural Sciences;2007-33
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