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《Journal of Anhui Agricultural Sciences》 2007-28
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Study on the Streptomycin Resistance Screening of High-yield Strain of Jinggangmycin

NIU Li-na et al (Key Laboratory of Bio-resources and Eco-environment,Ministry of Education,Sichuan University,Chengdu,Sichuan 610064)  
UV and nitrous acid complex mutagenesis was conducted on Streptomyces hygroscopicus var.Jingganggensis Yen SJ-6 produced Jinggangmycin.In the medium plate with streptomycin at minimum inhibiting concentration of strain spores,a lot of streptomycin resistant mutants were obtained by streptomycin resistance screening,including the mutant UN-80.Under the condition of shaking flask fermentation,the Jinggangmycin chemical titer reached 15 943 μg/ml,increased 84.4% than the original strain SJ-6.After continuously cultured for 5 generations the strain had basically stable chemical titer and better hereditary stability.
【CateGory Index】: S476.1
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