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《Journal of Anhui Agricultural Sciences》 2007-29
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Study on Technical Conditions for Degrading Corn Stalk by Compound Enzyme

CHEN He et al(College of Life Science and Engineering,Shaanxi University of Science and Technology,Xi'an,Shaanxi 710021)  
[Objective] The aim of the research was to seek the optimum technical conditions for enzyme hydrolysis of corn stalk.[Method] In the orthogonal test with 5 factors and 3 levels,compound enzyme was used for enzyme hydrolysis of corn stalk to study the optimum technical conditions for degrading corn stalk.Morphological and structural changes of corn stalk in degradation process were observed by scanning electron microscopy.[Result] The factors that affected corn stalk degradation by the compound enzyme were as follows: pH substrate concentration reaction time enzyme usage reaction temperature.The optimum condition of degrading corn stalk with the compound enzymes were: enzyme usage of 0.20 %,substrate concentration of 5.0 %,reaction time of 3.0 h,reaction temperature of 50 ℃ and pH of 5.0.Under this condition,the degradation rate was the highest(up to 27.6 %).Electron microscope observation showed that surface waxy structure of corn stalk was degraded after enzyme hydrolysis.And inner compact structure became loose and emerged inanition.[Conclusion] The research provided reference basis for biological utilization of corn stalk.
【Fund】: 咸阳市科技局重点项目(K0311-2)
【CateGory Index】: S38
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