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《Journal of Anhui Agricultural Sciences》 2007-29
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Detection of Total Flavonoids in Cell Culture Substance of Carpesium.macrocephalum Franch.et Sav

LI Yu-ping et al(College of Life Science,Northwest A & F University,Yangling,Shaanxi 712100)  
[Objective] The paper was conducted to take qualitative and quantitative detection of the total flavonoids in fruits and cell culture substance of Carpesium.macrocephalum Franch.et Sav.[Methods] Qualitative detection of the flavonoids in fruits and cell culture substance of Carpesium.macrocephalum was carried out by test tube method and UV spectrophotometry,while the content of total flavonoids was determined by UV spectrophotometry.[Result] There were flavonoids in fruit cell culture of Carpesium.macrocephalum by test tube method and UV spectrophotometry.The linear relation of the control was good within the range of 8.00~48.00 μg/mL at the maximum wavelength of 510 nm by test tube method and UV spectrophotometry(r=0.999 7).The average recovery was 98.60 % and RSD was 1.12 %(n=5).[Conclusion] The method was simple,accurate,stable,sensitive and had good reproducibility.It could be used to detect the total flavonoids in fruit and cell culture substance of Carpesium.macrocephalum.
【Fund】: 国家“十五”科技攻关项目(2002BA516A04)
【CateGory Index】: S567.239
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