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《Journal of Anhui Agricultural Sciences》 2007-30
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Sequence Analysis of Cyt b Gene of Haizi Buffalo

LI Shi-ping et al(College of Bioscience and Biotechnology,Yangzhou University,Yangzhou,Jiangsu 225009)  
The cytochrome b(Cyt b) gene complete sequences(1 140 bp) of 18 Haizi buffalos were sequenced and analyzed.Among 1 140 sites of Cyt b gene,10 variable sites were found,all of which were transitions.In 10 variable sites,only one site caused amino-acid replacement substitution,and the other were all synonymous substitutions.Sequence analysis showed that there were remarkable base composition bias and codon usage bias.The results also showed that there were two stop codons(AGA and AGG) in Cyt b gene in Haizi buffalo,and it was first reported that AGG was one of the stop codons in Cyt b gene in Bovidae.18 Cyt b gene sequences defined 4 haplotypes,and the haplotype diversity(Hd) and Nucleotide diversity(Pi) were 0.527 and 0.004 63 respectively,showing abundant genetic diversity in Haizi buffalo.The data also suggested that there were two or more matriline origins in Haizi buffalo.
【Fund】: 国家自然科学基金项目(30571323)
【CateGory Index】: Q953
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