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《Journal of Anhui Agricultural Sciences》 2007-30
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Screening Antagonistic Actinomycetes against Plant Pathogenic Fungi

ZHU Wei-ling et al(Department of Bioengineering and Pharmacy,Wuhan Technology College,Wuhan,Hubei 430023)  
The objective of the research was to screen antagonistic actinomycetes that had inhibition effects on plant pathogenic fungi.With cotton-wilt fusarium as target fungus,actinomycetes were separated from 4 soil samples by diluting flat method to screen antagonistic actinomycetes and determine their physical and chemical characteristics and antifungal spectrum.113 strains of actinomycetes were separated and 23 strains were antagonistic actinomycetes,in which 6 strains had strong resistance to cotton wilt fusarium.Except 332# strain,other strains could all liquefy glutin.Except 323# strain,other strains could all peptonize milk.6 strains of antagonistic actinomycetes all had strong capabilities of utilizing starch.Strains of 219# and 323# could decompound cellulose.The optimum growth temperature for strains of 121#,323#,327# and 430# was about 28 ℃,that of 219# and 332# strain were 28~35 ℃ and 35~40 ℃ respectively.The optimum pH value for growth of 6 strains were all 6~7.6 strains had strong inhibitory effect on cotton wilt fusarium.Strains of 121# and 219# had broad antifungal spectrum and obvious resistance to fungi.strains of 121#,327# and 430# grew well in preliminary liquid culture and produced higher antimicrobial active substances,which could be used in the research and application of antifungal metabolites.
【CateGory Index】: S476
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