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《Journal of Anhui Agricultural Sciences》 2007-31
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Effects of Multi-Factor on Efficiency of Polyploid Induction with Colchicine

CHANG Qing-yun et al(Key Laboratory for Genetic and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,Beijing Forestry University,Beijing 100083)  
[Objective] The aim of the research was to confirm the effects of each factor on the polyploid induction efficiency by colchicine.[Method] In orthogonal experiment,the effects of colchicine concentration,the duration and frequence of once ultraviolet radiation,the duration and frequence of once temperature fall on polyploid induction efficiency of Robinia pseudoacacia by colchicine were studied.[Result] The effect of ultraviolet radiation duration on the induction efficiency by colchicine was most significant with the highest swelling rate for 80 s,followed by the concentration.The swelling rates in different concentration groups showed the periodic change and it was slightly raised with the increasing of the concentration as a whole.The effects of radiation frequence and temperature fall frequence on the swelling rate were less with twice and once best.The temperature fall duration of 5 min was best.The induction effects of different radiation duration and different radiation time point had obvious differences.Once ultraviolet radiation couldn't induce the doubling of chromosome in root tip cell and couldn't reduce the minimum effective induction concentration of colchicine.An inhibition occurred at once dosage for 2 min,an obvious growth inhibition occurred at single dosage for 4 min and the growth stopped at single dosage for 8 min.[Conclusion] The optimum combination is 0.3% colchicine,radiation of 40 s for 2 times and temperature fall of 5 min for 1 time.
【Fund】: 国家“十一五”科技支撑计划专题(2006BAD01A1502)
【CateGory Index】: S33
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