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《Journal of Anhui Agricultural Sciences》 2007-35
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Effect of Trifolium Pratense Oligopeptide on Immune Function of Mouse

ZHANG Yong et al (College of Veterinary and Animal Sciences,Shenyang Agricultural University,Shenyang,Liaoning 110161)  
Objective The effect of oligopeptide extracted from Trifolium Pratense on growth and immune functions of mouse was studied.Method Sixty-four Kunming mice with body weights between 18 g and 25 g were divided into 4 groups and randomly assigned to 4 treatments of sixteen mice for each(eight males and eight females).Males and females were fed in separate cages.Three groups were supplementded with 5.0 mg/ml,1.0 mg/ml and 0.1 mg/ml oligopeptide solution respectively and one group was given normal saline as control.The solution volume for each mouse was 0.3 ml everyday.The experiment lasted for 21 days.Result Supplementation of oligopeptide increased weight gain(P0.05 and P0.01 for 1.0 mg/ml and 0.1 mg/ml groups respectively).The spleen weight of 0.1 mg/ml group was significantly higher than the control(P0.01).All experimental groups had higher thymus gland weight(P0.01 for 5.0 mg/ml group and P0.05 for the rest).Supplementation of oligopeptide increased hemolysin(P0.01 and P0.05 for 1.0 mg/ml and 0.1 mg/ml groups respectively).Right ear thickness of 0.1 mg/ml group increased(P0.05),indicating obvious delayed type hypersensitivity.The percentage of macrophage devouring chicken red blood cell increased(P0.01 for 1.0 mg/ml and 0.1 mg/ml groups and P0.05 for the rest).1.0 mg/ml group had a higher percentage of T lymphocyte than the control(P0.05).Conclusion Oligopeptide extracted from Trifolium Pratense could improve some immune functions of mouse.The oligopeptide solution at 1.0 mg/ml and 0.1 mg/ml with a volume of 0.3 ml everyday had much significant result.
【CateGory Index】: S541.2
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