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《Journal of Anhui Agricultural Sciences》 2007-35
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Research on the Technology of Extracting Total Flavonoids from Camellia Oleifera Leaves by Microwave Method

GONG Jian-liang et al (College of Materials Science and Engineering,Central South University of Forestry and Technology,Changsha,Hunan 410004)  
Objective The aim of the study was to provide some reference foundation for the high-efficient development and comprehensive utilization of Camellia oleifera resources.Method Total flavonoids were extracted from C.oleifera leaves by microwave method.The influences of the factors such as ethanol concentration,ratio of solid to liquid,extraction temperature,time and frequency on the yield of total flavonoids were discussed.The optimum technic condition for the extraction was confirmed through single-factor experiment and orthogonal experiment.Result In the single-factor experiments,the yield of total flavonoids from C.oleifera leaves increased firstly and then decreased along with the increment of time of microwave and ethanol concentration,increased along with the increment of temperature,but too high temperature would decrease the yield.The results from orthogonal experiment,variance analysis and significance test showed that the influence order of 4 factors on the yield of total flavonoids was ethanol concentration extraction temperature ratio of solid to liquid extraction time.The optimum technology condition of extracting total flavonoids from C.oleifera leaves by microwave method was with 50 % ethanol,ratio of solid to liquid of 1:20,temperature at 80 ℃,extraction time of 8 min and extraction times of 2,under which the yield of total flavonoids was 48.53 mg/g.Conclusion Microwave extraction can reduce producing cost effectively and enhance economic benefit.
【Fund】: 湖南省教育厅科研基金资助项目(07C811)
【CateGory Index】: S794.4
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