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《Journal of Anhui Agricultural Sciences》 2007-35
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Comparison of Determination Methods of Paris polyphylla var.yunnanensis Pollen Activity

WANG Ding-kang et al (Department of Biology,Kunming College,Kunming,Yunnan 650031)  
Objective The study aimed to increase the reproduction efficiency of Paris polyphylla var.yunnanensis.Method The pollens of dehisced anthors of flowering P.polyphylla plants was slightly painted on the slide glass,the activities of P.polyphylla pollens were determined resp.by iodine-potassium iodide dyeing method(I2-KI),triphenyl tetrazolium chloride staining method(TTC),aceto carmine dyeing method and fluorescein diacetate method with a little improvement.Result The dyeing rate of TTC method was lower,being only 27.51 %;that of aceto carmine dyeing method and fluorescein diacetate method were higher,being 91.30 % and 90.55 % resp.,and the determined data were close to each other.Although I2-KI method could dye pollens with dyeing rate of 94.80 %,the dyed pollens included degraded and inactive ones.Conclusion Aceto carmine dyeing method and fluorescein diacetate method has higher dyeing rate.The active and inactive pollens can be distinguished better and the viability of P.polyphylla pollens can be reflected more accurately.
【Fund】: 云南省白药集团资助项目
【CateGory Index】: S567.239
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